Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배상수 | - |
dc.date.accessioned | 2018-08-02T07:30:40Z | - |
dc.date.available | 2018-08-02T07:30:40Z | - |
dc.date.issued | 2016-07 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v. 6, Page. 1-7 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://www.nature.com/articles/srep30620 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/74300 | - |
dc.description.abstract | Microalgae are versatile organisms capable of converting CO2, H2O, and sunlight into fuel and chemicals for domestic and industrial consumption. Thus, genetic modifications of microalgae for enhancing photosynthetic productivity, and biomass and bio-products generation are crucial for both academic and industrial applications. However, targeted mutagenesis in microalgae with CRISPR-Cas9 is limited. Here we report, a one-step transformation of Chlamydomonas reinhardtii by the DNA-free CRISPR-Cas9 method rather than plasmids that encode Cas9 and guide RNAs. Outcome was the sequential CpFTSY and ZEP two-gene knockout and the generation of a strain constitutively producing zeaxanthin and showing improved photosynthetic productivity. | en_US |
dc.description.sponsorship | This work was supported by grants from Institute for Basic Science (IBS-R021-D1) to J.-S.K. the Korea CCS R&D Center (KCRC) (NRF-2014M1A8A1049273) to E.J. and the Plant Molecular Breeding Center of Next Generation BioGreen 21 Program (PJ01119201) to S.B. | en_US |
dc.language.iso | en | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | CAS9 RIBONUCLEOPROTEINS | en_US |
dc.subject | TARGET SITES | en_US |
dc.subject | HUMAN-CELLS | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | GENE | en_US |
dc.subject | ZEAXANTHIN | en_US |
dc.subject | NUCLEASES | en_US |
dc.subject | DELIVERY | en_US |
dc.subject | GUIDE | en_US |
dc.title | DNA-free two-gene knockout in Chlamydomonas reinhardtii via CRISPR-Cas9 ribonucleoproteins | en_US |
dc.type | Article | en_US |
dc.relation.volume | 6 | - |
dc.identifier.doi | 10.1038/srep30620 | - |
dc.relation.page | 1-7 | - |
dc.relation.journal | SCIENTIFIC REPORTS | - |
dc.contributor.googleauthor | Baek, Kwangryul | - |
dc.contributor.googleauthor | Kim, Duk Hyoung | - |
dc.contributor.googleauthor | Jeong, Jooyeon | - |
dc.contributor.googleauthor | Sim, Sang Jun | - |
dc.contributor.googleauthor | Melis, Anastasios | - |
dc.contributor.googleauthor | Kim, Jin-Soo | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.contributor.googleauthor | Bae, Sangsu | - |
dc.relation.code | 2016012537 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | sangsubae | - |
dc.identifier.researcherID | E-5324-2017 | - |
dc.identifier.orcid | http://orcid.org/0000-0003-3615-8566 | - |
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