Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina
- Title
- Oxaloacetate and malate production in engineered Escherichia coli by expression of codon-optimized phosphoenolpyruvate carboxylase2 gene from Dunaliella salina
- Author
- 진언선
- Keywords
- Dunaliella salina; Phosphoenolpyruvate carboxylase; Escherichia coli; Malate; Oxaloacetate; ENZYME; ACID
- Issue Date
- 2013-01
- Publisher
- Springer Science + Business Media
- Citation
- Bioprocess and Biosystems Engineering, January 2013, 36(1), p.127-131
- Abstract
- A new phosphoenolpyruvate carboxylase (PEPC) gene of Dunaliella salina is identified using homology analysis was conducted using PEPC gene of Chlamydomonas reinhardtii and Arabidopsis thaliana. Recombinant E. coli SGJS115 with increased production of malate and oxaloacetate was developed by introducing codon-optimized phosphoenolpyruvate carboxylase2 (OPDSPEPC2) gene of Dunaliella salina. E. coli SGJS115 yielded a 9.9 % increase in malate production. In addition, E. coli SGJS115 exhibited two times increase in the yield of oxaloacetate over the E. coli SGJS114 having identified PEPC2 gene obtained from Dunaliella salina.
- URI
- https://link.springer.com/article/10.1007/s00449-012-0759-4https://repository.hanyang.ac.kr/handle/20.500.11754/69612
- ISSN
- 1615-7591
- DOI
- 10.1007/s00449-012-0759-4
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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