Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김태욱 | - |
dc.date.accessioned | 2018-04-19T07:56:02Z | - |
dc.date.available | 2018-04-19T07:56:02Z | - |
dc.date.issued | 2012-03 | - |
dc.identifier.citation | Journal of Experimental Botany, 2012, 63(5), P.1823-1833 | en_US |
dc.identifier.issn | 0022-0957 | - |
dc.identifier.uri | https://academic.oup.com/jxb/article/63/5/1823/525127 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/69494 | - |
dc.description.abstract | A metabolic study revealed that 28-norcastasterone in Arabidopsis is synthesized from cholesterol via the late C-6 oxidation pathway. On the other hand, the early C-6 oxidation pathway was found to be interrupted because cholestanol is converted to 6-oxocholestanol, but further metabolism to 28-norcathasterone was not observed. The 6-oxoBRs were found to have been produced from the respective 6-deoxoBRs administered to the enzyme solution, thus indicating that these 6-oxoBRs are supplied from the late C-6 oxidation pathway. Heterologously expressed CYP85A1 and CYP85A2 in yeast catalysed this C-6 oxidation, with CYP85A2 being much more efficient than CYP85A1. Abnormal growth of det2 and dwf4 was restored via the application of 28-norcastasterone and closer precursors. Furthermore, det2 and dwf4 could not convert cholesterol to cholestanol and cholestanol to 6-deoxo-28-norcathasterone, respectively. It is, therefore, most likely that the same enzyme system is operant in the synthesis of both 28-norcastasterone and castasterone. In the presence of S-adenosyl-L-methionine, the cell-free enzyme extract catalysed the C-24 methylation of 28-norcastasterone to castasterone, although the conversion rates of 28-norteasterone to teasterone and 28-nortyphasterol to typhasterol were much lower; this suggests that 28-norcastasterone is the primary precursor for the generation of C-28-BRs from C-27-BRs. | en_US |
dc.description.sponsorship | The authors would like to thank Dr S Takatsuto at the Joetsu University of Education for providing the authentic BRs and Drs D Pompon and P Urban for providing the pYeDP60 and the WAT21 yeast strain. This work was supported by a grant from the Next-Generation Bio-Green21 Program (No. PJ007967) Rural Development Administration to S-KK and the National Research Foundation of Korea Grant funded by the Korean Government (NRF-2009-353-C00021) to S-HJ in the Chung-Ang University, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Oxford Univ Press | en_US |
dc.subject | Arabidopsis thaliana | en_US |
dc.subject | brassinosteroids | en_US |
dc.subject | C-27-BRs biosynthesis | en_US |
dc.subject | 28-norcastasterone | en_US |
dc.title | Biosynthesis of a cholesterol-derived brassinosteroid, 28-norcastasterone, in Arabidopsis thaliana | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 63 | - |
dc.identifier.doi | 10.1093/jxb/err354 | - |
dc.relation.page | 1823-1833 | - |
dc.relation.journal | JOURNAL OF EXPERIMENTAL BOTANY | - |
dc.contributor.googleauthor | Joo, S. H. | - |
dc.contributor.googleauthor | Kim, T. W. | - |
dc.contributor.googleauthor | Son, S. H. | - |
dc.contributor.googleauthor | Lee, W. S. | - |
dc.contributor.googleauthor | Yokota, T. | - |
dc.contributor.googleauthor | Kim, S. K. | - |
dc.relation.code | 2012205083 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | twgibio | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.