Experimental validation of the solvent-gradient simulated moving bed process for optimal separation of phenylalanine and tryptophan
- Title
- Experimental validation of the solvent-gradient simulated moving bed process for optimal separation of phenylalanine and tryptophan
- Author
- 문성용
- Keywords
- Phenylalanine; Tryptophan; Poly-4-vinylpyridine (PVP); Amino acid separation; Simulated moving bed
- Issue Date
- 2012-03
- Publisher
- Elsevier Science B.V., Amsterdam.
- Citation
- Process Biochemistry, Vol.47, No.3 [2012], p401-409
- Abstract
- A solvent-gradient simulated moving bed (SG-SMB) process for separation of two useful amino acids, phenylalanine and tryptophan, has been studied in previous researches, which confirmed that the SG-SMB outperformed the corresponding isocratic SMB for the same separation task. However, all of the previous studies on the SG-SMB for separation of the two amino acids have been limited to process simulation and optimization, including some batch-chromatography tests. The experimental validation of such an SG-SMB process was attempted in this article. This task began by assembling the experimental unit of the SG-SMB process. Its operating conditions were determined from the SG-SMB optimization tool based on genetic algorithm, in which the mass-transfer parameters as well as the adsorption isotherm parameters of the feed components (two amino acids) were entered as the mathematical models expressed as a function of liquid-phase composition (i.e., modifier concentration in the liquid phase). Based on the determined operating conditions, the relevant SG-SMB experiment was conducted until a cyclic steady-state was reached. The assay of all the resultant product samples verified that the SG-SMB separation of interest was performed successfully as designed. The experimental data were also found to agree closely with the model predictions.
- URI
- http://www.sciencedirect.com/science/article/pii/S1359511311004089http://hdl.handle.net/20.500.11754/67690
- ISSN
- 1359-5113
- DOI
- 10.1016/j.procbio.2011.11.011
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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