Immobilizing Reporters for Molecular Imaging of the Extracellular Microenvironment in Living Animals
- Title
- Immobilizing Reporters for Molecular Imaging of the Extracellular Microenvironment in Living Animals
- Author
- 김영필
- Keywords
- LASER-SCANNING MICROSCOPY; RESONANCE ENERGY-TRANSFER; IN-VIVO; MATRIX METALLOPROTEINASES; TUMOR MICROENVIRONMENT; TISSUE INHIBITOR; CANCER; CELLS; INFLAMMATION; ACTIVATION
- Issue Date
- 2011-12
- Publisher
- AMERICAN CHEMICAL SOCIETY
- Citation
- ACS CHEMICAL BIOLOGY, 2011, 6(10), P.1117-1126
- Abstract
- We report here an immobilization strategy using a collagen,binding protein to deliver and confine synthetic reporters to the extracellular microenvironment in vivo for noninvasively imaging the activity of targets in the microenvironment. We show that the immobilization of reporters on collagens in the local microenvironment is highly efficient and physiologically stable for repetitive, long-term imaging. By using this strategy we successfully developed an immobilized bioluminescent activatable reporter and a dual-modality reporter to map and quantitatively image the activity of extracellular matrix metalloproteinases (MMP) in tumor-bearing mice. The inhibition of MMP activity by chemical inhibitor was also demonstrated in living subjects. We further demonstrated the general applicability of this immobilization strategy by imaging MMP activity at the inflammation site in a mouse model. Our results show that the in vivo immobilization of reporters can be used as a general strategy for probing the local extracellular microenvironment.
- URI
- https://pubs.acs.org/doi/abs/10.1021/cb200135ehttp://hdl.handle.net/20.500.11754/65805
- ISSN
- 1554-8929
- DOI
- 10.1021/cb200135e
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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