An albumin nanocomplex-based endosomal pH-activatable on/off probe system
- Title
- An albumin nanocomplex-based endosomal pH-activatable on/off probe system
- Author
- 최한곤
- Keywords
- Albumin; Nanocomplex; pH-sensitivity; Self-assembly; Tumor targeting; Zinc-imidazole interaction
- Issue Date
- 2016-07
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- COLLOIDS AND SURFACES B-BIOINTERFACES, v. 144, Page. 327-334
- Abstract
- Albumin has gained considerable interest as a material for fabricating nanoparticulate systems due to its biomedical advantages, such as biocompatibility and chemical functionality. Here, we report a new pH-sensitive albumin nanocomplex prototype with a zinc-imidazole coordination bond. Albumin was conjugated with 1-(3-aminopropyl)imidazole and mPEG(10kDa)-NHS, and the resulting albumin conjugate (PBI) was then modified with either Cy5.5 or BHQ-3. The newly formed albumin nanocomplex (C/BQ-PBI Zn NCs: similar to 116 nm) system was facilely self-assembled around pH 7.4 in the presence of Zn2+, but it quickly disassembled in an acidic environment (similar to pH 5.0). Based on this pH-sensitivity, C/BQ-PBI Zn NCs emitted strong near-infrared fluorescence and released Zn2+, turning "off" at pH similar to 7.4 (e.g., plasma) and "on" at pH similar to 5.0 (e.g., endo/lysosomes in tumor cells) on account of fluorescence resonance energy transfer. C/BQ-PBI Zn NCs displayed significant cytotoxicity due to an increase in cellular. Zn2+ in response to endosomal pH (similar to 5.0) in breast cancer MCF-7 cells and lung adenocarcinoma A549 cells. Particularly, confocal laser scanning microscopic images showed a strong fluorescence signal caused by the disassembly of C/BQ-PBI Zn NCs in the endosomal region of MCF-7 cells. Based on these results, we believe that this albumin nanocomplex is an attractive biocompatible tumor targeting probe carrier for the theranostic purpose. (C) 2016 Elsevier B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S092777651630279Xhttp://hdl.handle.net/20.500.11754/65736
- ISSN
- 0927-7765; 1873-4367
- DOI
- 10.1016/j.colsurfb.2016.04.022
- Appears in Collections:
- COLLEGE OF PHARMACY[E](약학대학) > PHARMACY(약학과) > Articles
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