Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이영식 | - |
dc.date.accessioned | 2018-04-10T02:04:53Z | - |
dc.date.available | 2018-04-10T02:04:53Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.citation | SCIENTIFIC REPORTS, v. 6, Article no. 23103 | en_US |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | https://www.nature.com/articles/srep23103 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/65535 | - |
dc.description.abstract | In human mesenchymal stem cells (hMSCs), toll-like receptor 3 (TLR3) and TLR4 act as key players in the tissue repair process by recognizing their ligands and stimulating downstream processes including cytokine release. The mechanisms of TLR3-and TLR4-mediated cytokine releases from hMSCs remain uncertain. Here, we show that exposure to the TLR3 agonist polyinosinic-polycytidylic acid (poly(I:C)) or incubation with the TLR4 agonist lipopolysaccharide (LPS) increased the mRNA expression levels of TLR3, TLR4 and cytokines in hMSCs. Poly(I:C) exposure rather than LPS incubation not only elevated inositol 1,4,5-triphosphate receptor (IP3R) expression and IP3R-mediated Ca2+ release, but also promoted Orai and STIM expression as well as store-operated Ca2+ entry into hMSCs. In addition, we also observed that 21 Ca2+ signaling genes were significantly up-regulated in response to TLR3 priming of hMSCs by RNA sequencing analysis. Both poly(I: C) and LPS exposure enhanced cytokine release from hMSCs. The enhanced cytokine release vanished upon siRNA knockdown and chelation of intracellular Ca2+. These data demonstrate that TLR3-and TLR4-priming differentially enhance Ca2+ signaling and cytokine expression, and Ca2+ -dependently potentiates cytokine release in hMSCs. | en_US |
dc.description.sponsorship | This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) grant funded by the Ministry of Education (NRF-2013R1A1A2010992) and the Korea government (MSIP) (No. 2011-0030049). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | NATURE PUBLISHING GROUP | en_US |
dc.subject | MESENCHYMAL STEM-CELLS | en_US |
dc.subject | TOLL-LIKE RECEPTORS | en_US |
dc.subject | INNATE IMMUNITY | en_US |
dc.subject | FUNCTIONAL EXPRESSION | en_US |
dc.subject | CALCIUM-CHANNELS | en_US |
dc.subject | STROMAL CELLS | en_US |
dc.subject | MODULATION | en_US |
dc.subject | APOPTOSIS | en_US |
dc.subject | ENTRY | en_US |
dc.subject | LIPOPOLYSACCHARIDE | en_US |
dc.title | TLR3-/4-Priming Differentially Promotes Ca2+ Signaling and Cytokine Expression and Ca2+ -Dependently Augments Cytokine Release in hMSCs | en_US |
dc.type | Article | en_US |
dc.relation.volume | 6 | - |
dc.identifier.doi | 10.1038/srep23103 | - |
dc.relation.page | 1-13 | - |
dc.relation.journal | SCIENTIFIC REPORTS | - |
dc.contributor.googleauthor | Park, KS | - |
dc.contributor.googleauthor | Kim, S.H | - |
dc.contributor.googleauthor | Das, A | - |
dc.contributor.googleauthor | Yang, SN | - |
dc.contributor.googleauthor | Jung, KH | - |
dc.contributor.googleauthor | Kim, MK | - |
dc.contributor.googleauthor | Berggren, PO | - |
dc.contributor.googleauthor | Lee, Y | - |
dc.contributor.googleauthor | Chai, JC | - |
dc.contributor.googleauthor | Kim, HJ | - |
dc.relation.code | 2016012537 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MOLECULAR AND LIFE SCIENCE | - |
dc.identifier.pid | yslee | - |
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