Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이수재 | - |
dc.date.accessioned | 2018-03-26T01:42:20Z | - |
dc.date.available | 2018-03-26T01:42:20Z | - |
dc.date.issued | 2014-07 | - |
dc.identifier.citation | Aquatic toxicology, 2014, 152, P.308-317 | en_US |
dc.identifier.issn | 0166-445X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0166445X14001568?via%3Dihub | - |
dc.description.abstract | In this study, we investigated the effects of the water-accommodated fraction (WAF) of crude oil on the development and reproduction of the intertidal copepod Tigriopus japonicus through life-cycle experiments. Furthermore, we investigated the mechanisms underlying the toxic effects of WAF on this benthic organism by studying expression patterns of cytochrome P450 (CYP) genes. Development of T. japonicus was delayed and molting was interrupted in response to WAF exposure. Hatching rate was also significantly reduced in response to WAF exposure. Activities of antioxidant enzymes such as glutathione S-transferase (GST), glutathione reductase (GR), and catalase (CAT) were increased by WAF exposure in a concentration-dependent manner. These results indicated that WAF exposure resulted in oxidative stress, which in turn was associated with dysfunctional development and reproduction. To evaluate the involvement of cytochrome P450 (CYP) genes, we cloned the entire repertoire of CYP genes in T. japonicus (n = 52) and found that the CYP genes belonged to five different clans (i.e., Clans 2, 3, 4, mitochondrial, and 20). We then examined expression patterns of these 52 CYP genes in response to WAF exposure. Three TJ-CYP genes (CYP3024A2, CYP3024A3, and CYP3027C2) belonging to CYP clan 3 were significantly induced by WAF exposure in a time- and concentration-dependent manner. We identified aryl hydrocarbon responsive elements (AhRE), xenobiotic responsive elements (XREs), and metal response elements (MRE) in the promoter regions of these three CYP genes, suggesting that these genes are involved in detoxification of toxicants. Overall, our results indicate that WAF can trigger oxidative stress and thus induce dysfunctional development and reproduction in the copepod T. japonicus. Furthermore, we identified three TJ-CYP genes that represent potential biomarkers of oil pollution. (C) 2014 Elsevier B.V. All rights reserved. | en_US |
dc.description.sponsorship | We thank Prof. H.-U. Dahms for his comments and two anony-mous reviewers for their valuable comments on the manuscript,and also thank Dr. W. J. Shim (KIOST) for his help in determiningthe chemical composition of WAF. This study was supported bya research fund (Oil Spill Environmental Impact Assessment andEnvironmental Restoration; PM57431) from the Ministry of Oceansand Fisheries. | en_US |
dc.publisher | Elsevier Science B.V., Amsterdam. | en_US |
dc.subject | Water accommodated fraction | en_US |
dc.subject | Oxidative stress | en_US |
dc.subject | Copepod | en_US |
dc.subject | Tigriopus japonicus | en_US |
dc.subject | Cytochrome P450 | en_US |
dc.subject | CYP | en_US |
dc.title | Crude oil exposure results in oxidative stress-mediated dysfunctional development and reproduction in the copepod Tigriopus japonicus and modulates expression of cytochrome P450 (CYP) genes | en_US |
dc.type | Article | en_US |
dc.relation.volume | 152 | - |
dc.identifier.doi | 10.1016/j.aquatox.2014.04.027 | - |
dc.relation.page | 308-317 | - |
dc.relation.journal | AQUATIC TOXICOLOGY | - |
dc.contributor.googleauthor | Han, Jeonghoon | - |
dc.contributor.googleauthor | Won, Eun-Ji | - |
dc.contributor.googleauthor | Hwang, Dae-Sik | - |
dc.contributor.googleauthor | Shin, Kyung-Hoon | - |
dc.contributor.googleauthor | Lee, Yong Sung | - |
dc.contributor.googleauthor | Leung, Kenneth Mei-Yee | - |
dc.contributor.googleauthor | Lee, Su-Jae | - |
dc.contributor.googleauthor | Lee, Jae-Seong | - |
dc.relation.code | 2014025389 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | sj0420 | - |
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