Characterization of bacteriophage phi Pto-bp6g, a novel phage that lyses Pseudomonas tolaasii causing brown blotch disease in mushrooms
- Title
- Characterization of bacteriophage phi Pto-bp6g, a novel phage that lyses Pseudomonas tolaasii causing brown blotch disease in mushrooms
- Author
- 윤문영
- Keywords
- teriophage; Brown blotch disease; Genome analysis; Pseudomonas tolaasii
- Issue Date
- 2012-12
- Publisher
- ELSEVIER SCIENCE BV
- Citation
- Journal of Microbiological Methods, 2012, 91(3), P.514-519
- Abstract
- The bacteriophage, phi Pto-bp6g, exhibited strong bactericidal activity against Pseudomonas tolaasii, the bacterium that causes brown blotch disease in cultivated mushrooms. Analysis of phage morphology with an electron microscope revealed that phi Pto-bp6g contains an icosahedral head and a long tail, which is classified as the family of Siphoviridae. The phage was observed to lyse P. tolaasii in the broth about 4 h after inoculation, indicating a putative lyric pathway exists during bacterial growth. The whole genome of phi Pto-bp6g was completely sequenced, with a length of 26,499 bp and a G + C content of 42.7%. A total of 77 open reading frames (ORFs) as putative coding sequences were identified and annotated, whereas 43 ORFs possessed no homologs. Proteins of several ORFs showed similarity with proteins of a diverse group of phages, including Siphoviridae (5 ORFs), Myoviridae (11 ORFs), and Podoviridae (4 ORFs). Phage proteins were grouped into three categories based on their predicted functions: (i) DNA replication and nucleotide metabolism, (ii) phage particle formation, and (iii) host interaction. Since there is no identified gene encoding integrase and toxins in phage genome, phage phi Pto-bp6g could be potentially applicable as a safe biological control reagent against brown blotch disease in mushroom cultivation. (c) 2012 Elsevier B.V. All rights reserved.
- URI
- https://www.sciencedirect.com/science/article/pii/S016770121200320X?via%3Dihubhttp://hdl.handle.net/20.500.11754/50971
- ISSN
- 0167-7012
- DOI
- 10.1016/j.mimet.2012.09.032
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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