Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김영미 | - |
dc.date.accessioned | 2018-03-20T08:16:34Z | - |
dc.date.available | 2018-03-20T08:16:34Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.citation | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1011, Page. 108-113 | en_US |
dc.identifier.issn | 1570-0232 | - |
dc.identifier.issn | 1873-376X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S157002321530386X | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49809 | - |
dc.description.abstract | A preparative separation method using consecutive sample injection centrifugal partition chromatography (CPC) was developed to obtain sesamin and sesamolin from defatted sesame meal extracts. A two-phase solvent system consisting of n-hexane–ethyl acetate–methanol–water (8:2:8:2, v/v) was applied in reversed-phase mode (descending mode). Preliminary experiments with an SCPC-100 (column volume: 100 mL) were performed to select the appropriate two-phase solvent system and sample injection times; these parameters were then used with an SCPC-1000 (column volume: 1000 mL) in a 10-fold scale-up preparative run. A sample containing 3 g of crude extract was consecutively injected four times onto the SCPC-1000, which yielded 328 mg of sesamin and 168 mg of sesamolin. These compounds were analyzed by high-performance liquid chromatography and determined to have purities of 95.6% and 93.9%, respectively. Sesamin and sesamolin (30 μM) increased antioxidant response element (ARE) luciferase activity 2.6-fold and 1.9-fold, respectively. | en_US |
dc.description.sponsorship | This study was supported by a grant from the Korean Health Technology R&D Project, Ministry of Health and Welfare, Republic of Korea (HI14C0018). | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCIENCE BV | en_US |
dc.subject | Centrifugal partition chromatography | en_US |
dc.subject | Consecutive sample injection | en_US |
dc.subject | Sesamin | en_US |
dc.subject | Sesamolin | en_US |
dc.subject | Antioxidant response element | en_US |
dc.title | Preparative separation of sesamin and sesamolin from defatted sesame meal via centrifugal partition chromatography with consecutive sample injection | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.jchromb.2015.12.062 | - |
dc.relation.page | 108-113 | - |
dc.relation.journal | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | - |
dc.contributor.googleauthor | Jeon, Je-Seung | - |
dc.contributor.googleauthor | Park, Chae Lee | - |
dc.contributor.googleauthor | Syed, Ahmed Shah | - |
dc.contributor.googleauthor | Kim, Young-Mi | - |
dc.contributor.googleauthor | Cho, Il Je | - |
dc.contributor.googleauthor | Kim, Chul Young | - |
dc.relation.code | 2016001027 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | ymikim12 | - |
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