Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최제민 | - |
dc.date.accessioned | 2018-03-19T09:17:44Z | - |
dc.date.available | 2018-03-19T09:17:44Z | - |
dc.date.issued | 2014-12 | - |
dc.identifier.citation | MOLECULAR BIOLOGY REPORTS, 권: 41, 호: 12, 페이지: 8117-8126 | en_US |
dc.identifier.issn | 0301-4851 | - |
dc.identifier.issn | 1573-4978 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs11033-014-3711-7 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49149 | - |
dc.description.abstract | Cytokines are released from the cell, bind to their receptors, and affect cellular responses. The precursor form of interleukin 1 alpha (pIL-1 alpha) has a nuclear localization sequence (NLS) that causes it to be localized to the nucleus and regulate specific gene expression. The amino acids of the NLS are basic amino acid-rich sequences, as is the cell penetrating peptide (CPP), which has been widely studied as a way to deliver macromolecules into cells. Here, we hypothesized that the NLS in pIL-1 alpha (pIL-1 alpha NLS) can penetrate the cell membrane and it could deliver macromolecules such as protein in vivo. We characterized cell membrane penetration ability of pIL-1 alpha NLS or its tandem repeated form (2pIL-1 alpha NLS) to enhance its intracellular delivery efficiency. 2pIL-1 alpha NLS showed comparable protein delivery efficiency to TAT-CPP and it mediates endocytosis following heparan sulfate interaction. 2pIL-1 alpha NLS conjugated enhanced green fluorescence protein was localized to the nucleus and the cytoplasm. Intra-peritoneal administration of 2pIL-1 alpha NLS conjugated dTomato protein showed remarkable in vivo intracellular delivery efficiency in various tissues including spleen, liver, and intestine in mice. Moreover, cytotoxicity of 2pIL-1 alpha NLS was not observed even at 100 mu M. Our results demonstrate cell membrane-penetrating function of NLS in pIL-1 alpha, which can be used as a safe therapeutic macromolecular delivery peptide. | en_US |
dc.description.sponsorship | This study is supported by the Basic Science Research Program through the Grants of National Research Foundation of Korea (NRF-2013R1A1A2A10060048) and Hanyang University grant (HY-2011-00000001004). | en_US |
dc.language.iso | en | en_US |
dc.publisher | SPRINGER | en_US |
dc.subject | IL-1 alpha | en_US |
dc.subject | Cytokine | en_US |
dc.subject | Nuclear localization sequence | en_US |
dc.subject | Cell penetrating peptide | en_US |
dc.title | Cell membrane penetrating function of the nuclear localization sequence in human cytokine IL-1 alpha | en_US |
dc.type | Article | en_US |
dc.relation.no | 12 | - |
dc.relation.volume | 41 | - |
dc.identifier.doi | 10.1007/s11033-014-3711-7 | - |
dc.relation.page | 8117-8126 | - |
dc.relation.journal | MOLECULAR BIOLOGY REPORTS | - |
dc.contributor.googleauthor | Koo | - |
dc.contributor.googleauthor | Yoon, Heeseok | - |
dc.contributor.googleauthor | Kim, Won-Ju | - |
dc.contributor.googleauthor | Lim, Sangho | - |
dc.contributor.googleauthor | Park, Hong-Jai | - |
dc.contributor.googleauthor | Choi, Je-Min | - |
dc.relation.code | 2014036185 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | jeminchoi | - |
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