Gene Therapy Using Hepatocyte Growth Factor Expressing Adenovirus Improves Skin Flap Survival in a Rat Model
- Title
- Gene Therapy Using Hepatocyte Growth Factor Expressing Adenovirus Improves Skin Flap Survival in a Rat Model
- Author
- 윤채옥
- Keywords
- Hepatocyte Growth Factor; Adenovirus; Gene Therapy; Skin Flap
- Issue Date
- 2014-11
- Publisher
- KOREAN ACAD MEDICAL SCIENCES, 302 75 DONG DU ICHON, DONG YONGSAN KU, SEOUL 140 031, SOUTH KOREA
- Citation
- JOURNAL OF KOREAN MEDICAL SCIENCE, 권: 29, 페이지: S228-S236
- Abstract
- Hepatocyte growth factor (HGF) is a potent angiogenic factor that can stimulate the production of blood vessels in ischemic tissue. We investigated whether gene therapy using HGF-expressing adenovirus could enhance skin flap survival. Sprague-Dawley rats were randomly divided into three groups. Rats were subdermally injected with HGF-expressing adenovirus (HGF virus group), recombinant HGF (rhHGF group), or phosphate buffered saline (PBS group) 2 days before and immediately after 3 x 9 cm caudal flap elevation. The survival area of the skin flap, the ratio of blood flow, CD31-positive vessels and, VEGF expression were examined. Skin flap viability was significantly increased in the HGF virus group compared to the rhHGF and PBS groups (71.4%+/- 5.9%, 63.8%+/- 6.4%, and 39.2%+/- 13.0%, respectively) (P=0.025). Furthermore, the blood flow ratio was significantly increased in the HGF virus group. In the HGF virus group, the number of CD31-positive vessels and vascular endothelial growth factor (VEGF) expression were significantly increased. Gene therapy using HGF-expressing adenovirus increase VEGF expression, the number of viable capillaries, and blood flow to the flap, thereby improving skin flap survival.
- URI
- https://www.synapse.koreamed.org/search.php?where=aview&id=10.3346/jkms.2014.29.S3.S228&code=0063JKMS&vmode=FULLhttp://hdl.handle.net/20.500.11754/49058
- ISSN
- 1011-8934; 1598-6357
- DOI
- 10.3346/jkms.2014.29.S3.S228
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > BIOENGINEERING(생명공학과) > Articles
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- jkms-29-S228.pdfDownload
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