Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2018-03-19T07:55:35Z | - |
dc.date.available | 2018-03-19T07:55:35Z | - |
dc.date.issued | 2014-11 | - |
dc.identifier.citation | JOURNAL OF KOREAN MEDICAL SCIENCE, 권: 29, 페이지: S228-S236 | en_US |
dc.identifier.issn | 1011-8934 | - |
dc.identifier.issn | 1598-6357 | - |
dc.identifier.uri | https://www.synapse.koreamed.org/search.php?where=aview&id=10.3346/jkms.2014.29.S3.S228&code=0063JKMS&vmode=FULL | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/49058 | - |
dc.description.abstract | Hepatocyte growth factor (HGF) is a potent angiogenic factor that can stimulate the production of blood vessels in ischemic tissue. We investigated whether gene therapy using HGF-expressing adenovirus could enhance skin flap survival. Sprague-Dawley rats were randomly divided into three groups. Rats were subdermally injected with HGF-expressing adenovirus (HGF virus group), recombinant HGF (rhHGF group), or phosphate buffered saline (PBS group) 2 days before and immediately after 3 x 9 cm caudal flap elevation. The survival area of the skin flap, the ratio of blood flow, CD31-positive vessels and, VEGF expression were examined. Skin flap viability was significantly increased in the HGF virus group compared to the rhHGF and PBS groups (71.4%+/- 5.9%, 63.8%+/- 6.4%, and 39.2%+/- 13.0%, respectively) (P=0.025). Furthermore, the blood flow ratio was significantly increased in the HGF virus group. In the HGF virus group, the number of CD31-positive vessels and vascular endothelial growth factor (VEGF) expression were significantly increased. Gene therapy using HGF-expressing adenovirus increase VEGF expression, the number of viable capillaries, and blood flow to the flap, thereby improving skin flap survival. | en_US |
dc.description.sponsorship | This research was supported by a National Research Foundation of Korea (NRF) grant funded by the Korea government (MEST) (No. 2009-0075307, DK Rah), (No. 2012-0008180, WJ Lee), a grant from the Ministry of Knowledge Economy (10030051, Dr. C-O Yun), and grants from the Korea Science and Engineering Foundation (R15-2004-024-02001-0, 2009K001644, 2010-0029220, Dr. C-O. Yun). | en_US |
dc.language.iso | en | en_US |
dc.publisher | KOREAN ACAD MEDICAL SCIENCES, 302 75 DONG DU ICHON, DONG YONGSAN KU, SEOUL 140 031, SOUTH KOREA | en_US |
dc.subject | Hepatocyte Growth Factor | en_US |
dc.subject | Adenovirus | en_US |
dc.subject | Gene Therapy | en_US |
dc.subject | Skin Flap | en_US |
dc.title | Gene Therapy Using Hepatocyte Growth Factor Expressing Adenovirus Improves Skin Flap Survival in a Rat Model | en_US |
dc.type | Article | en_US |
dc.relation.volume | 29 | - |
dc.identifier.doi | 10.3346/jkms.2014.29.S3.S228 | - |
dc.relation.page | 228-236 | - |
dc.relation.journal | JOURNAL OF KOREAN MEDICAL SCIENCE | - |
dc.contributor.googleauthor | Rah, Dong Kyun | - |
dc.contributor.googleauthor | Yun, In Sik | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.contributor.googleauthor | Bin Lee, Sae | - |
dc.contributor.googleauthor | Lee, Won Jai | - |
dc.relation.code | 2014033608 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | DEPARTMENT OF BIOENGINEERING | - |
dc.identifier.pid | chaeok | - |
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