Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이수재 | - |
dc.date.accessioned | 2018-03-15T07:01:33Z | - |
dc.date.available | 2018-03-15T07:01:33Z | - |
dc.date.issued | 2012-11 | - |
dc.identifier.citation | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, Nov 2012, 30(5), P.1180-1186 | en_US |
dc.identifier.issn | 1107-3756 | - |
dc.identifier.uri | https://www.spandidos-publications.com/10.3892/ijmm.2012.1115 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/47306 | - |
dc.description.abstract | Hypoxia is a common feature of tumors that occurs across a wide variety of malignancies. Multiple myeloma is an incurable malignant disorder of plasma cells in the bone marrow. Although bone marrow hypoxia is crucial for normal hematopoiesis, the effect of hypoxia on multiple myeloma is poorly understood. In this study, we demonstrated that cobalt chloride (CoCl2)-mediated hypoxia decreased cell viability and altered gene expression in U266 human multiple myeloma cells. CoCl2 induced the loss of cell viability in a concentration-dependent manner. In addition, FACS analysis revealed that the loss of cell viability was related to apoptosis. Using microarray analysis, we identified mRNA expression profile changes in response to CoCl2 treatment in U266 cells. Four hundred and fifty-two mRNAs exhibited >2-fold changes in expression in CoCl2-treated U266 cells compared to their expression in control cells. A follow-up bioinformatics study revealed that a great number of genes with altered expression were involved in apoptosis, cell cycle, transcription and development. In conclusion, these results provide novel evidence that CoCl2-mediated hypoxia affects the expression profiles of genes that are functionally related to apoptosis and angiogenesis in U266 multiple myeloma cells. | en_US |
dc.description.sponsorship | We are grateful to all members of our research group for their support and advice regarding this study. This study resulted from the Konkuk University Research Support Program. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Spandidos Publications | en_US |
dc.subject | hypoxia | en_US |
dc.subject | multiple myeloma | en_US |
dc.subject | cell cycle arrest | en_US |
dc.subject | cobalt chloride | en_US |
dc.subject | gene expression | en_US |
dc.title | The hypoxia-mimetic agent cobalt chloride induces cell cycle arrest and alters gene expression in U266 multiple myeloma cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 5 | - |
dc.relation.volume | 30 | - |
dc.identifier.doi | 10.3892/ijmm.2012.1115 | - |
dc.relation.page | 1180-1186 | - |
dc.relation.journal | INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | - |
dc.contributor.googleauthor | Bae, Seung-Hee | - |
dc.contributor.googleauthor | Jeong, Hye-Jung | - |
dc.contributor.googleauthor | Cha, Hwa-Jun | - |
dc.contributor.googleauthor | Kim, Ka-Ram | - |
dc.contributor.googleauthor | Choi, Yeong-Min | - |
dc.contributor.googleauthor | An, In-Sook | - |
dc.contributor.googleauthor | Koh, Hyea-Jung | - |
dc.contributor.googleauthor | Lim, Dae-Jin | - |
dc.contributor.googleauthor | Lees, Su-Jae | - |
dc.contributor.googleauthor | An, Sung-Kwan | - |
dc.relation.code | 2012212713 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | sj0420 | - |
dc.identifier.researcherID | 8066538700 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.