Glutaredoxin 2a, a mitochondrial isoform, plays a protective role in a human cell line under serum deprivation
- Title
- Glutaredoxin 2a, a mitochondrial isoform, plays a protective role in a human cell line under serum deprivation
- Author
- 최호진
- Keywords
- Glutaredoxin; Reactive oxygen species; Serum deprivation; Apoptosis
- Issue Date
- 2012-04
- Publisher
- Springer Science + Business Media
- Citation
- MOLECULAR BIOLOGY REPORTS, 2012, 39(4), P.3755-3765
- Abstract
- The roles of mitochondrial glutaredoxin (Grx2a) under serum deprivation were assessed using the human stable HepG2 cell lines overexpressing or down-regulating Grx2a. The Grx2a-overexpressing stable cells displayed enhanced proliferation, decreased reactive oxygen species (ROS) and caspase-3 activity levels, and increased total GSH level, compared to the vector control cells. These characteristics of the overexpressing stable cells were reversed by down-regulating Grx2a in the same cell line. In the limited serum conditions, the Grx2a-overexpressing stable pcDNA3.0/HA-Grx2a cells exhibited higher cellular viabilities and total GSH level, and showed much lower enhancement in ROS and caspase-3 activity levels than the vector control pcDNA3.0/HA cells. However, the Grx2a-down-regulating stable cells gave rise to diminished cellular viabilities and further decreased total GSH level, and contained significantly higher ROS and caspase-3 activity levels, under serum deprivation than the vector control cells. These results suggest that Grx2a plays proliferative and anti-apoptotic roles under serum deprivation.
- URI
- http://link.springer.com/article/10.1007%2Fs11033-011-1152-0http://hdl.handle.net/20.500.11754/46944
- ISSN
- 0301-4851
- DOI
- 10.1007/s11033-011-1152-0
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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