Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김철근 | - |
dc.date.accessioned | 2018-03-14T06:12:32Z | - |
dc.date.available | 2018-03-14T06:12:32Z | - |
dc.date.issued | 2014-01 | - |
dc.identifier.citation | Journal of Animal Science, Volume 92, Issue 1, Pages 106?118, | en_US |
dc.identifier.issn | 0021-8812 | - |
dc.identifier.issn | 1525-3163 | - |
dc.identifier.uri | https://academic.oup.com/jas/article/92/1/106/4701852 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/46662 | - |
dc.description.abstract | Undifferentiated germ cells have the capacity to develop into sperm capable of fertilizing oocytes and contributing genetic material to subsequent generations. The most primitive prepubertal undifferentiated germ cells include gonocytes and undifferentiated spermatogonia, including spermatogonial stem cells (SSC). Gonocytes, present in the testis at birth, differentiate into SSC, which maintain spermatogenesis for the remainder of the male's life. Because of their capacity to contribute to lifelong spermatogenesis, undifferentiated germ cells are attractive targets for genetic modification to produce transgenic animals, including cattle. To maximize the efficiency of genetic modification of bovine gonocytes and SSC, effective enrichment techniques need to be developed. Selection of bovine gonocytes using differential plating was improved 8-fold (P < 0.001) when using a combination of extracellular matrix proteins, including laminin, fibronectin, collagen type IV, and gelatin, compared to using laminin and gelatin alone. Selected cells labeled with PKH26 formed colonies of donor-derived germ cells after transplantation into recipient mouse testes, indicating putative stem cell function. Significantly more colonies (P < 0.001) per 1 × 105 viable transplanted cells were formed from isolated nonadherent cells (203 ± 23.2) compared to adherent (20 ± 2.7) or Percoll (45.5 ± 4.5) selected cells. After selection, some gonocytes were transduced using a lentiviral vector containing the transgene for the enhanced green fluorescent protein. Transduction efficiency was 17%. Collectively, these data demonstrate effective methods for the selection and genetic modification of bovine undifferentiated germ cells. | en_US |
dc.description.sponsorship | This research was supported by the Korea Healthcare technology R&D Project, Ministry of Health and Welfare, Republic of Korea (Grant A120080) and the Next-Generation BioGreen 21 Program (Grant PJ009554), Rural Development Administration, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | American Society of Animal Science | en_US |
dc.subject | Physiology | en_US |
dc.subject | Endocrinology | en_US |
dc.subject | Reproduction | en_US |
dc.title | Lentiviral modification of enriched populations of bovine male gonocytes | en_US |
dc.type | Article | en_US |
dc.relation.volume | 92 | - |
dc.identifier.doi | 10.2527/jas.2013-6885 | - |
dc.relation.page | 106-118 | - |
dc.relation.journal | JOURNAL OF ANIMAL SCIENCE | - |
dc.contributor.googleauthor | Kim, K.-J. | - |
dc.contributor.googleauthor | Cho, C. M. | - |
dc.contributor.googleauthor | Kim, B.-G. | - |
dc.contributor.googleauthor | Lee, Y.-A. | - |
dc.contributor.googleauthor | Kim, B.-J. | - |
dc.contributor.googleauthor | Kim, Y.-H. | - |
dc.contributor.googleauthor | Kim, C. G. | - |
dc.contributor.googleauthor | Schmidt, J. A. | - |
dc.contributor.googleauthor | Ryu, B.-Y. | - |
dc.relation.code | 2014032326 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | cgkim | - |
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