PCDHB14- and GABRB1-like nervous system developmental genes are altered during early neuronal differentiation of NCCIT cells treated with ethanol
- Title
- PCDHB14- and GABRB1-like nervous system developmental genes are altered during early neuronal differentiation of NCCIT cells treated with ethanol
- Author
- 이영식
- Keywords
- Ethanol; folic acid; FASD; nervous system; transcriptomic; NEURAL STEM-CELLS; RETINOIC ACID; IN-VITRO; CORTICAL-NEURONS; EXPRESSION; EXPOSURE; ALCOHOL; PROTEIN; RECEPTORS; MICE
- Issue Date
- 2015-12
- Publisher
- SAGE PUBLICATIONS LTD
- Citation
- HUMAN & EXPERIMENTAL TOXICOLOGY, v. 34, No. 10, Page. 1017-1027
- Abstract
- Ethanol (EtOH) exposure during embryonic development causes dysfunction of the central nervous system (CNS). Here, we examined the effects of chronic EtOH on gene expression during early stages of neuronal differentiation. Human embryonic carcinoma (NCCIT) cells were differentiated into neuronal precursors/lineages in the presence or absence of EtOH and folic acid. Gene expression profiling and pathway analysis demonstrated that EtOH deregulates many genes and pathways that are involved in early brain development. EtOH exposure downregulated several important genes, such as PCDHB14, GABRB1, CTNND2, NAV3, RALDH1, and OPN5, which are involved in CNS development, synapse assembly, synaptic transmission, and neurotransmitter receptor activity. GeneGo pathway analysis revealed that the deregulated genes mapped to disease pathways that were relevant to fetal alcohol spectrum disorders (FASD, such as neurotic disorders, epilepsy, and alcohol-related disorders). In conclusion, these findings suggest that the impairment of the neurological system or suboptimal synapse formation resulting from EtOH exposure could underlie the neurodevelopmental disorders in individuals with FASD.
- URI
- http://journals.sagepub.com/doi/abs/10.1177/0960327114566827http://hdl.handle.net/20.500.11754/46397
- ISSN
- 0960-3271; 1477-0903
- DOI
- 10.1177/0960327114566827
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > MOLECULAR AND LIFE SCIENCE(분자생명과학과) > Articles
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML