Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이재성 | - |
dc.date.accessioned | 2018-03-12T04:34:47Z | - |
dc.date.available | 2018-03-12T04:34:47Z | - |
dc.date.issued | 2013-08 | - |
dc.identifier.citation | Marine Environmental Research, Sep 2013, 90, P.75-84 | en_US |
dc.identifier.issn | 0141-1136 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0141113613000974?via%3Dihub | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/45297 | - |
dc.description.abstract | To report a novel CYP genes and to evaluate its potency as a biomarker for oil pollution, we cloned three CYP genes and measured their expression profiles under controlled lab conditions using real-time reverse transcription PCR (real-time RT-PCR) after exposure of the water accommodated fraction (WAF) of Iranian crude oil and benzo[α]pyrene (B[α]P) as a positive control. Of these, CYP432A1 (CYP3 clan) gene was significantly induced by B[α]P exposure, indicating that the CYP3 clan gene would play an important role in polycyclic aromatic hydrocarbon (PAH) metabolisms, particularly for B[α]P in this species. However, the Perinereis nuntia CYP431A1 mRNA, a CYP2 clan gene, was sensitively expressed to WAF exposure with other two CYP genes. As one of Phase II detoxification enzymes, the glutathione S-transferase (GST) genes also upregulated with other antioxidant genes (SOD and CAT), indicating that WAF-exposed P. nuntia was properly responding to this kind of chemical stress. Thus, three CYP genes from the polychaete, P. nuntia have a potential as a biomarker in monitoring of the marine sediment after an oil spill accident. | en_US |
dc.description.sponsorship | We thank Dr. Hans-U. Dahms for his comments on the manuscript. We would like to express our sincere thanks to Drs. K. Ra and K.-T. Kim (KIOST) for their technical assistance. This work was supported by a grant (PM11090) funded to Jae-Seong Lee from the Ministry of Land, Transport and Maritime Affairs and also supported by a grant (No. 2009-0067801) funded to Young-Mi Lee from the National Research Foundation. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Polychaete | en_US |
dc.subject | Perinereis nuntia | en_US |
dc.subject | Cytochrome P450Water accommodated fraction | en_US |
dc.subject | WAFBenzo[α]pyrene | en_US |
dc.subject | Antioxidant genes | en_US |
dc.title | Expression of three novel cytochrome P450 (CYP) and antioxidative genes from the polychaete, Perinereis nuntia exposed to water accommodated fraction (WAF) of Iranian crude oil and Benzo[alpha]pyrene | en_US |
dc.type | Article | en_US |
dc.relation.volume | 90 | - |
dc.identifier.doi | 10.1016/j.marenvres.2013.05.014 | - |
dc.relation.page | 75-84 | - |
dc.relation.journal | MARINE ENVIRONMENTAL RESEARCH | - |
dc.contributor.googleauthor | Won, E.J. | - |
dc.contributor.googleauthor | Rhee, J.S. | - |
dc.contributor.googleauthor | Shin, K.H. | - |
dc.contributor.googleauthor | Jung, J.H. | - |
dc.contributor.googleauthor | Shim, W.J. | - |
dc.contributor.googleauthor | Lee, Y.M. | - |
dc.contributor.googleauthor | Lee, J.S. | - |
dc.relation.code | 2013011202 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF CHEMISTRY | - |
dc.identifier.pid | JSLee2 | - |
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