Silaffin Peptides as a Novel Signal Enhancer for Gravimetric Biosensors
- Title
- Silaffin Peptides as a Novel Signal Enhancer for Gravimetric Biosensors
- Author
- 상병인
- Keywords
- Silaffin peptides; Biomimetic silica formation; R5 peptide; Quartz crystal microbalances; Signal enhancer
- Issue Date
- 2013-05
- Publisher
- Springer
- Citation
- Applied Biochemistry and Biotechnology, 2013, 170(1), P.25-31
- Abstract
- Application of biomimetic silica formation to gravimetric biosensors has been conducted for the first time. As a model system, silaffin peptides fused with green fluorescent protein (GFP) were immobilized on a gold quartz crystal resonator for quartz crystal microbalances using a self-assembled monolayer. When a solution of silicic acid was supplied, silica particles were successfully deposited on the Au surface, resulting in a significant change in resonance frequency (i.e., signal enhancement) with the silaffin?GFP. However, frequency was not altered when bare GFP was used as a control. The novel peptide enhancer is advantageous because it can be readily and quantitatively conjugated with sensing proteins using recombinant DNA technology. As a proof of concept, this study shows that the silaffin domains can be employed as a novel and efficient biomolecular signal enhancer for gravimetric biosensors.
- URI
- https://link.springer.com/article/10.1007%2Fs12010-013-0161-yhttp://hdl.handle.net/20.500.11754/44703
- ISSN
- 0273-2289; 1559-0291
- DOI
- 10.1007/s12010-013-0161-y
- Appears in Collections:
- COLLEGE OF ENGINEERING[S](공과대학) > CHEMICAL ENGINEERING(화학공학과) > Articles
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