Expression of superoxide dismutase (SOD) genes from the copper-exposed polychaete, Neanthes succinea
- Title
- Expression of superoxide dismutase (SOD) genes from the copper-exposed polychaete, Neanthes succinea
- Author
- 이재성
- Keywords
- Polychaete; Neanthes succinea; Copper and zinc superoxide dismutase; Manganese superoxide dismutase; Oxidative stress; Copper
- Issue Date
- 2011-05
- Publisher
- Elsevier Science B.V., Amsterdam.
- Citation
- MARINE POLLUTION BULLETIN, Vol.63 No.5-12 [2011], 277-286
- Abstract
- To evaluate the superoxide dismutase (SOD) genes as a potential indicator of environmental stress, we cloned and sequenced the full-length cDNA of a Cu/Zn-SOD and Mn-SOD genes from the marine polychaete, Neanthes succinea. Ns-Cu/Zn- and Mn-SOD expression was measured using quantitative real-time RT-PCR in response to the oxidative stress induced by copper in the form of CuCl2. Ns-Cu/Zn-SOD expression increased in a dose dependent manner but the increase of Mn-SOD expression was evident only at a high concentration of copper. Particularly, Ns-Mn-SOD dramatically increased in the 72μg/L CuCl2-exposed groups, indicating that both Ns-SOD genes were induced by copper exposure. These results suggest that Ns-Cu/Zn- and Ns-Mn-SOD would play important roles in the detoxification of cellular oxidative stresses in the polychaete, N. succinea. Furthermore, as part of field work, the marine polychaete, N. succineaCu/Zn-SOD mRNA collected from the polluted area was accordingly upregulated in expression but not for NS-Mn-SOD.
- URI
- http://www.sciencedirect.com/science/article/pii/S0025326X11002256?via%3Dihub
- ISSN
- 0025-326X
- DOI
- 10.1016/j.marpolbul.2011.04.023
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > CHEMISTRY(화학과) > Articles
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