Secondary Ions Mass Spectrometric Signal Enhancement of Peptides on Enlarged-Gold Nanoparticle Surfaces
- Title
- Secondary Ions Mass Spectrometric Signal Enhancement of Peptides on Enlarged-Gold Nanoparticle Surfaces
- Author
- 김영필
- Keywords
- TOF-SIMS; PROTEIN
- Issue Date
- 2012-04
- Publisher
- AMER CHEMICAL SOC, 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
- Citation
- Analytical chemistry, 84, 11, 4784 - 4788
- Abstract
- A high surface coverage of gold nanoparticles (AuNPs) is a prerequisite for enhancing the peptide signal intensity in time-of-flight secondary ion mass spectrometry (TOF-SIMS). Here, we demonstrate the TOF-SIMS signal amplification of peptides on a surface by enlarging surface-confined AuNPs using the NH2OH/Au3+ seeding method. Because of the increased surface area and spherical structure of the Au, the SIMS intensity of the peptides became significantly enhanced on the enlarged-AuNPs surface, especially at high concentrations of peptide solution (>10 mu M), compared to that of the bare gold surface or submonolyer of AuNPs. We are confident that this will be a useful method for diagnosis and bioassay with high sensitivity in a label-free manner.
- URI
- https://pubs.acs.org/doi/ipdf/10.1021/ac300336hhttp://hdl.handle.net/20.500.11754/37862
- ISSN
- 0003-2700
- DOI
- 10.1021/ac300336h
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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