Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김철근 | - |
dc.date.accessioned | 2018-02-15T02:25:01Z | - |
dc.date.available | 2018-02-15T02:25:01Z | - |
dc.date.issued | 2011-05 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH, 권: 39, 호: 16, 페이지: 6944-6955 | en_US |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://eds.b.ebscohost.com/eds/detail/detail?vid=0&sid=f45619af-fa41-4836-95c0-24b424c9e5a1%40sessionmgr120&bdata=Jmxhbmc9a28mc2l0ZT1lZHMtbGl2ZQ%3d%3d#AN=000294556800016&db=edswsc | - |
dc.identifier.uri | https://academic.oup.com/nar/article/39/16/6944/2411224 | - |
dc.description.abstract | GATA-1 and NF-E2 are erythroid specific activators that bind to the beta-globin locus. To explore the roles of these activators in transcription of the human fetal stage specific gamma-globin genes, we reduced GATA-1 and p45/NF-E2 using shRNA in erythroid K562 cells. GATA-1 or p45/NF-E2 knockdown inhibited the transcription of the gamma-globin genes, hypersensitive site (HS) formation in the LCR and chromatin loop formation of the beta-globin locus, but histone acetylation across the locus was decreased only in the case of GATA-1 knockdown. In p45/NF-E2 knockdown cells, GATA-1 binding was maintained at the LCR HSs and gamma-globin promoter, but NF-E2 binding at the LCR HSs was reduced by GATA-1 knockdown regardless of the amount of p45/NF-E2 in K562 cells. These results indicate that histone acetylation is dependent on GATA-1 binding, but the binding of GATA-1 is not sufficient for the gamma-globin transcription, HS formation and chromatin loop formation and NF-E2 is required. This idea is supported by the distinctive binding pattern of CBP and Brg1 in the beta-globin locus. Furthermore GATA-1-dependent loop formation between HS5 and 3'HS1 suggests correlation between histone modifications and chromatin looping. | en_US |
dc.description.sponsorship | Grant of the Korea Healthcare Technology R&D Project, Ministry for Health, Welfare & Family Affairs, Republic of Korea (A090172). Funding of open access charge: Pusan National University, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | OXFORD UNIV PRESS, GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND | en_US |
dc.subject | LOCUS-CONTROL REGION | en_US |
dc.subject | RNA-POLYMERASE-II | en_US |
dc.subject | CHROMATIN-STRUCTURE | en_US |
dc.subject | HISTONE ACETYLATION | en_US |
dc.subject | QUANTITATIVE-ANALYSIS | en_US |
dc.subject | HYPERSENSITIVE SITES | en_US |
dc.subject | REGULATORY REGIONS | en_US |
dc.subject | DELETION MUTATIONS | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | MOUSE | en_US |
dc.title | The distinctive roles of erythroid specific activator GATA-1 and NF-E2 in transcription of the human fetal gamma-globin genes | en_US |
dc.type | Article | en_US |
dc.relation.no | 16 | - |
dc.relation.volume | 39 | - |
dc.identifier.doi | 10.1093/nar/gkr253 | - |
dc.relation.page | 6944-6955 | - |
dc.relation.journal | NUCLEIC ACIDS RESEARCH | - |
dc.contributor.googleauthor | Kim, Yea Woon | - |
dc.contributor.googleauthor | Kim, Seoyeon | - |
dc.contributor.googleauthor | Kim, AeRi | - |
dc.contributor.googleauthor | Kim, Chul Geun | - |
dc.relation.code | 2011207178 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | cgkim | - |
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