Transcriptomic Analysis of Haematococcus lacustris during Astaxanthin Accumulation under High Irradiance and Nutrient Starvation
- Title
- Transcriptomic Analysis of Haematococcus lacustris during Astaxanthin Accumulation under High Irradiance and Nutrient Starvation
- Author
- 진언선
- Keywords
- Haematococcus lacustris; astaxanthin; cDNA microarray; transcriptomics; high irradiance; nutrient starvation
- Issue Date
- 2011-06
- Publisher
- KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING, KOREAN SCIENCE TECHNOLOGY CENTER, #704 YEOGSAM-DONG, KANGNAM-KU, SEOUL 135-703, SOUTH KOREA
- Citation
- BIOTECHNOLOGY AND BIOPROCESS ENGINEERING; AUG 2011, 16 4, p698-p705, 8p.
- Abstract
- In this study, a cDNA microarray was developed from over 60,000 mRNA readings to analyze the expression profiles of transcriptomes of Haematococcus lacustris under astaxanthin-inducing culture conditions, high irradiance and nitrate starvation. Among 20,033 genes on the cDNA microarray, 2,675 genes exhibited a twofold or greater difference in expression. Of these, 1,333 genes were up-regulated and 1,342 genes were down-regulated. A significant decrease in the expression of chlorophyll biosynthesis and light harvesting complex (LHC) related genes were observed under astaxanthin inducing conditions (forming red cyst cells). On the other hand, respiration-related genes, lipid metabolism-related genes and stress response-related genes were activated in the red cyst cells under stress conditions. These results enabled a better understanding of the cell responses during stress induction of H. lacustris such as photosynthesis, respiration, and some biopathways.
- URI
- http://link.springer.com/article/10.1007%2Fs12257-011-0081-zhttp://hdl.handle.net/20.500.11754/36117
- ISSN
- 1226-8372
- DOI
- 10.1007/s12257-011-0081-z
- Appears in Collections:
- COLLEGE OF NATURAL SCIENCES[S](자연과학대학) > LIFE SCIENCE(생명과학과) > Articles
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