Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
- Title
- Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration
- Author
- 황경균
- Keywords
- Bone regeneration; Cell adhesion; Cell proliferation; Membrane; Osteoblasts; Silk fibroin
- Issue Date
- 2016-03
- Publisher
- 대한악안면성형재건외과학회
- Citation
- Maxillofacial Plastic Reconstructive Surgery, v. 38, NO 03, Page. 1-6
- Abstract
- Background The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR). Methods Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining. Results Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05). Conclusions These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.
- URI
- https://jkamprs.springeropen.com/articles/10.1186/s40902-016-0062-4http://hdl.handle.net/20.500.11754/34255
- ISSN
- 2288-8586
- DOI
- 10.1186/s40902-016-0062-4
- Appears in Collections:
- COLLEGE OF MEDICINE[S](의과대학) > MEDICINE(의학과) > Articles
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