Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 황경균 | - |
dc.date.accessioned | 2018-01-08T06:38:04Z | - |
dc.date.available | 2018-01-08T06:38:04Z | - |
dc.date.issued | 2016-03 | - |
dc.identifier.citation | Maxillofacial Plastic Reconstructive Surgery, v. 38, NO 03, Page. 1-6 | en_US |
dc.identifier.issn | 2288-8586 | - |
dc.identifier.uri | https://jkamprs.springeropen.com/articles/10.1186/s40902-016-0062-4 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/34255 | - |
dc.description.abstract | Background The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR). Methods Various cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining. Results Optical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05). Conclusions These results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR. | en_US |
dc.description.sponsorship | This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (NRF-2013R1A1A2009975). This work was supported by a grant from the Next-Generation BioGreen 21 Program (No.PJ01121404), Rural Development Administration, Republic of Korea. | en_US |
dc.language.iso | en | en_US |
dc.publisher | 대한악안면성형재건외과학회 | en_US |
dc.subject | Bone regeneration | en_US |
dc.subject | Cell adhesion | en_US |
dc.subject | Cell proliferation | en_US |
dc.subject | Membrane | en_US |
dc.subject | Osteoblasts | en_US |
dc.subject | Silk fibroin | en_US |
dc.title | Cell attachment and proliferation of osteoblast-like MG63 cells on silk fibroin membrane for guided bone regeneration | en_US |
dc.type | Article | en_US |
dc.relation.no | 03 | - |
dc.relation.volume | 38 | - |
dc.identifier.doi | 10.1186/s40902-016-0062-4 | - |
dc.relation.page | 1-6 | - |
dc.relation.journal | Maxillofacial Plastic Reconstructive Surgery | - |
dc.contributor.googleauthor | Yoo, Chae-Kyung | - |
dc.contributor.googleauthor | Jeon, Jae-Yun | - |
dc.contributor.googleauthor | Kim, You-Jin | - |
dc.contributor.googleauthor | Kim, Seong-Gon | - |
dc.contributor.googleauthor | Hwang, Kyung-Gyun | - |
dc.relation.code | 2016018986 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF MEDICINE[S] | - |
dc.sector.department | DEPARTMENT OF MEDICINE | - |
dc.identifier.pid | hkg | - |
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