Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 엄애선 | - |
dc.contributor.author | Su San Na Kang | - |
dc.date.accessioned | 2017-11-29T02:29:32Z | - |
dc.date.available | 2017-11-29T02:29:32Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/33417 | - |
dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000430918 | en_US |
dc.description.abstract | This study aimed to compare the sensitivity of porcine-specific primers targeting different mitochondrial DNA for the detection of porcine gelatin in ramen stock powder designated “Halal” using SYBR Green I real-time PCR. Four mitochondrial DNA markers (D-loop, ATP8, ND5, and 12S rRNA) were used to detect the porcine gelatin DNA. Extraction of DNA from porcine gelatin and spiked mixtures was successfully performed using a DNeasy Mericon Food Kit. Each primer had high porcine specificity for D-loop, ATP8, and ND5 genes, but not the 12S rRNA gene. Also, the detection limit for each primer was tested in a serial dilution of porcine gelatin DNA (10, 1, 0.1, 0.05, 0.01, and 0.005 ng) and ramen stock powder spiked with porcine gelatin (1, 0.1, 0.05, and 0.025%). Assays targeting both D-loop and ND5 genes were able to detect a level as low as 0.01 ng, while the detection limit for the ATP8 gene was 0.05 ng in pure porcine gelatin. The detection limits of D-loop, ATP8, and ND5 genes were 0.05%, 0.1%, and 1% (w/w) in ramen stock powder spiked with porcine gelatin, respectively. In conclusion, a novel detection system targeting the mitochondrial D-loop was superior to the others and could be an effective and simple method to authenticate Halal products. | - |
dc.publisher | 한양대학교 | - |
dc.title | Development and comparison of a porcine gelatin detection system targeting mitochondrial markers for Halal authentication | - |
dc.type | Theses | - |
dc.contributor.googleauthor | 강수산나 | - |
dc.sector.campus | S | - |
dc.sector.daehak | 대학원 | - |
dc.sector.department | 식품영양학과 | - |
dc.description.degree | Master | - |
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