Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 진언선 | - |
dc.date.accessioned | 2017-08-31T00:27:08Z | - |
dc.date.available | 2017-08-31T00:27:08Z | - |
dc.date.issued | 2015-11 | - |
dc.identifier.citation | ACS APPLIED MATERIALS & INTERFACES, v. 7, NO 49, Page. 27554-27561 | en_US |
dc.identifier.issn | 1944-8244 | - |
dc.identifier.uri | http://pubs.acs.org/doi/10.1021/acsami.5b09964 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/28779 | - |
dc.description.abstract | Superior green algal cells showing high lipid production and rapid growth rate are considered as an alternative for the next generation green energy resources. To achieve the biomass based energy generation, transformed microalgae with superlative properties should be developed through genetic engineering. Contrary to the normal cells, microalgae have rigid cell walls, so that target gene delivery into cells is challengeable. In this study, we report a ZnO nanowire-incorporated microdevice for a high throughput microalgal transformation. The proposed microdevice was equipped with not only a ZnO nanowire in the microchannel for gene delivery into cells but also a pneumatic poIydimethylsiloxane (PDMS) microvalve to modulate the cellular attachment and detachment from the nanowire. As a model, hygromycin B resistance gene cassette (Hyg3) was functionalized on the hydrothermally grown ZnO nanowires through a disulfide bond and released into green algal cells, Chlamydomonas reinhardtii, by reductive cleavage. During Hyg3 gene delivery, a monolithic PDMS membrane was bent down, so that algal cells were pushed down toward ZnO nanowires. The supply of vacuum in the pneumatic line made the PDMS membrane bend up, enabling the gene delivered algal cells to be recovered from the outlet of the microchannel. We successfully confirmed Hyg3 gene integrated in microalgae by amplifying the inserted gene through polymerase chain reaction (PCR) and DNA sequencing. The efficiency of the gene delivery to algal cells using the ZnO nanowire-incorporated microdevice was 6.52 X 10(4)- and 9.66 X 10(4)-fold higher than that of a traditional glass bead beating and electroporation. | en_US |
dc.description.sponsorship | This work was supported by the Korea CCS R&D Center (KCRC) grant funded by the Korea government (Ministry of Science, ICT & Future Planning; 2014M1A8A1049278) and the Engineering Research Center of Excellence Program of Korea Ministry of Science, ICT & Future Planning (MSIP)/National Research Foundation of Korea (NRF; 2014R1A5A1009799). | en_US |
dc.language.iso | en | en_US |
dc.publisher | AMER CHEMICAL SOC | en_US |
dc.subject | ZnO nanowire | en_US |
dc.subject | microalgal | en_US |
dc.subject | gene delivery | en_US |
dc.subject | transformation | en_US |
dc.subject | high throughput | en_US |
dc.subject | microfluidics | en_US |
dc.subject | biofuel | en_US |
dc.title | Exogenous Gene Integration for Microalgal Cell Transformation Using a Nanowire-Incorporated Microdevice | en_US |
dc.type | Article | en_US |
dc.relation.no | 49 | - |
dc.relation.volume | 7 | - |
dc.identifier.doi | 10.1021/acsami.5b09964 | - |
dc.relation.page | 27554-27561 | - |
dc.relation.journal | ACS APPLIED MATERIALS & INTERFACES | - |
dc.contributor.googleauthor | Bae, Sunwoong | - |
dc.contributor.googleauthor | Park, Seunghye | - |
dc.contributor.googleauthor | Kim, Jung | - |
dc.contributor.googleauthor | Choi, Jong Seob | - |
dc.contributor.googleauthor | Kim, Kyung Hoon | - |
dc.contributor.googleauthor | Kwon, Donguk | - |
dc.contributor.googleauthor | Jin, EonSeon | - |
dc.contributor.googleauthor | Park, Inkyu | - |
dc.contributor.googleauthor | Kim, Do Hyun | - |
dc.contributor.googleauthor | Seo, Tae Seok | - |
dc.relation.code | 2015001547 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | esjin | - |
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