Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신인철 | - |
dc.date.accessioned | 2017-06-08T01:38:16Z | - |
dc.date.available | 2017-06-08T01:38:16Z | - |
dc.date.issued | 2015-09 | - |
dc.identifier.citation | CELLULAR SIGNALLING, v. 27, NO 9, Page. 1882-1894 | en_US |
dc.identifier.issn | 0898-6568 | - |
dc.identifier.issn | 1873-3913 | - |
dc.identifier.uri | http://www.sciencedirect.com/science/article/pii/S0898656815001503 | - |
dc.identifier.uri | http://hdl.handle.net/20.500.11754/27679 | - |
dc.description.abstract | CD44 was recently identified as a cancer initiation marker on the cell membrane. The cytoplasmic tail of CD44 is known to bind ERM (ezrin, radixin, moesin) proteins, cytoskeletal proteins like ankyrin, and the non-receptor tyrosine kinase c-Src. CD44 transmits its oncogenic signaling via c-Src and its downstream effectors. To investigate the role of CD44 in breast cancer cells, we generated CD44 knock-down cells via retroviral delivery of shRNA against CD44. We found that silencing of CD44 decreased the proliferation, migration, and invasion of breast cancer cells. The expression and activity of cell migration-related proteins, including c-Src, paxillin, and FAK were decreased by CD44 silencing. We also found that the c-Jun protein level was negatively regulated via induction of a GSK-3 beta-dependent degradation pathway in CD44 knock-down cells. The expression level of Sp1, a target gene product of c-Jun, was also decreased in these cells. Finally, CD44 knock-down suppressed both mRNA and protein levels of c-Sic and its downstream MAPK pathway as a result of down-regulation of Sp1 as a transcription factor for c-Src. Collectively, these results indicate that biological changes induced by CD44 silencing are mediated by cumulative down-regulation of c-Jun, Sp1, and c-Src in human breast cancer cells. (C) 2015 Elsevier Inc All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by NRF grant (2013-59143) from the Korea Research Foundation and the Converging Research Center Program funded by the Ministry of Science, ICT & Future Planning (Project No. 2014048814). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE INC | en_US |
dc.subject | CD44 | en_US |
dc.subject | c-Src | en_US |
dc.subject | c-Jun | en_US |
dc.subject | GSK-3 beta | en_US |
dc.subject | Breast cancer | en_US |
dc.title | CD44 regulates cell proliferation, migration, and invasion via modulation of c-Src transcription in human breast cancer cells | en_US |
dc.type | Article | en_US |
dc.relation.no | 9 | - |
dc.relation.volume | 27 | - |
dc.identifier.doi | 10.1016/j.cellsig.2015.05.002 | - |
dc.relation.page | 1882-1894 | - |
dc.relation.journal | CELLULAR SIGNALLING | - |
dc.contributor.googleauthor | Nam, KeeSoo | - |
dc.contributor.googleauthor | Oh, Sunhwa | - |
dc.contributor.googleauthor | Lee, Kyung-min | - |
dc.contributor.googleauthor | Yoo, Seung-ah | - |
dc.contributor.googleauthor | Shin, Incheol | - |
dc.relation.code | 2015001314 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF NATURAL SCIENCES[S] | - |
dc.sector.department | DEPARTMENT OF LIFE SCIENCE | - |
dc.identifier.pid | incheol | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.