Preventive effects of the butanol fraction of Justicia procumbens L. against dexamethasone-induced muscle atrophy in C2C12 myotubes
- Title
- Preventive effects of the butanol fraction of Justicia procumbens L. against dexamethasone-induced muscle atrophy in C2C12 myotubes
- Author
- 김재용
- Keywords
- Justicia procumbens L.; Muscle atrophy; C2C12; Dexamethasone; Protein degradation
- Issue Date
- 2022-11-22
- Publisher
- ELSEVIER SCI LTD
- Citation
- HELIYON, v. 8, no 11, article no. 11597, page. 1-9
- Abstract
- Skeletal muscle atrophy is associated with many diseases including cancer, inflammatory diseases, neuromuscular diseases, and acute critical illness. Justicia procumbens L. has been used as a herbal remedy, but the pharmaco-logical effect of J. procumbens on muscle atrophy has not yet been reported. Herein, we investigate the anti -atrophic effect of the n-butanol fraction of J. procumbens (JPBuFr) on dexamethasone (DEX)-induced muscle at-rophy in C2C12 myotubes. The myotubes diameter, MHC positive area, ROS production, and mitochondria contents were observed under a fluorescence microscope, and various proteins related to degradation or synthesis were analyzed by western blots. JPBuFr significantly attenuated a reduction of myotube diameter, mitochondrial content, ATP level, myosin heavy chain, and myogenin expression induced by DEX. Furthermore, co-treatment of DEX and JPBuFr not only increased phosphorylation of Akt, mTOR, and p70S6K proteins but also decreased reactive oxygen species production and expression of protein degradation factors (MuRF1, Atrogin-1, FoxO3a) compared to DEX treatment. These results suggest that JPBuFr may provide potential protective effects against muscle atrophy, giving it potential for the development of anti-atrophic health functional foods.
- URI
- https://www.sciencedirect.com/science/article/pii/S2405844022028857?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/191113
- ISSN
- 2405-8440
- DOI
- https://doi.org/10.1016/j.heliyon.2022.e11597
- Appears in Collections:
- RESEARCH INSTITUTE[E](부설연구소) > INSTITUTE OF PHARMACEUTICAL SCIENCE AND TECHNOLOGY(약학기술연구소) > Articles
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