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Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 조용우 | - |
| dc.contributor.author | 최영찬 | - |
| dc.date.accessioned | 2024-03-01T07:30:50Z | - |
| dc.date.available | 2024-03-01T07:30:50Z | - |
| dc.date.issued | 2021.2 | - |
| dc.identifier.uri | http://hanyang.dcollection.net/common/orgView/200000485686 | en_US |
| dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/188173 | - |
| dc.description.abstract | Biopharmaceuticalsareadrugthatusescells,proteins,geneticmaterials,andcomplexesfrombiologicaltissuesasatreatmentfordiseases.Andbiopharmaceuticalshavebeeninthespotlightasatherapeutictoolforincurablediseasestoreplaceexistingchemicaldrugs.Comparedtoconventionalchemicaldrugs,biopharmaceuticalshavedistinguishedcharacteristicssuchaslargermolecularweight(morethan150kDa),complexmolecularstructureandformulation.Complicatedandheterogeneousbiopharmaceuticalformulationrequirescomplexandrepetitiverefiningproceduresforproductbiopharmaceuticals,resultinginsignificantdifficultyandcostincreaseinproduction.Besides,understandingthecorrelationbetweenproductqualityandprocessparametersisstillbeingleaked.However,therearemanycasesinwhichclinicaltrialsforbiopharmaceuticalshavereportedtheamazingefficacyofvariousdiseases,thereforebiopharmaceuticalsareinthespotlightasanewtreatmentplatformforovercomingincurablediseases. Herein,theisolationandfunctionalizationofstemcellexosomesandextracellularmatrix(ECM)derivedfromadiposetissueswerestudiedandevaluatedonabasisofqualitybydesign(QbD)conception.Asarawmaterial,humanandporcineadiposetissuescaneasilybeobtainedthroughahugeamountofbiologicalwastes.thestemcellexosomes,whichareoneofthemainfactorsintheparacrineactionofstemcelltherapy,wereextractedfromhumanadipose-derivedstemcells(hASCs),andstemcellexosomeswereeffectivelyproducedthroughthetangentialflowfiltration(TFF)andsize-exclusionchromatographyprocess,wherearecompatibletomass-productionandgoodmanufacturingpractice(GMP).Ievaluatedthecorrelationbetweenprocessparametersandthequalityofstemcellexosomes,andthisallowedthemanufactureofhigh-purifiedstemcellexosomeswithanti-inflammatoryeffects.ECMwasisolatedfromhumanandporcineadiposetissueandtheECMwasmodifiedbymechanicalandbiochemicalprocessestoenhancetheirsafetyandfunctionality.ThesefunctionalECMcouldbeappliedtohemostatandadiposetissueregeneration.Moreover,themanufacturingprocessofbiopharmaceuticalsderivedfrombiologicalresourcescouldbeoptimizedandtheprocessoptimizationwasachievedbyunderstandingthecorrelationbetweenproductperformancesandprocessconditions.Moreover,thesestudiesofprocessdevelopmentforbiopharmaceuticalscouldbeusedasapracticalexampleoftheQbDbasedprocessdevelopmentforbiopharmaceuticals. | - |
| dc.publisher | 한양대학교 | - |
| dc.title | QualitybyDesign(QbD)BasedProcessDevelopmentforCell-FreeTherapeuticsUsingExosomeandExtracellularMatrix(ECMs)-DerivedfromAdiposeTissue | - |
| dc.title.alternative | 지방유래엑소좀및세포외기질을이용한무세포치료제의QbD기반생산공정개발 | - |
| dc.type | Theses | - |
| dc.contributor.googleauthor | Choi,YoungChan | - |
| dc.contributor.alternativeauthor | 최영찬 | - |
| dc.sector.campus | S | - |
| dc.sector.daehak | 대학원 | - |
| dc.sector.department | 바이오나노학과 | - |
| dc.description.degree | Doctor | - |
- Appears in Collections:
- GRADUATE SCHOOL[S](대학원) > BIONANOTECHNOLOGY(바이오나노학과) > Theses (Ph.D.)
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