A Bre1-associated Protein, Large 1 (Lge1), Promotes H2B Ubiquitylation during the Early Stages of Transcription Elongation
- Title
- A Bre1-associated Protein, Large 1 (Lge1), Promotes H2B Ubiquitylation during the Early Stages of Transcription Elongation
- Author
- 안성훈
- Keywords
- RNA-POLYMERASE-II; UBIQUITINATION; YEAST; HISTONE H2B; TERMINAL DOMAIN; REGULATES H3 METHYLATION; SAGA COMPLEX; SACCHAROMYCES-CEREVISIAE; SYSTEMATIC IDENTIFICATION; MASS-SPECTROMETRY
- Issue Date
- 2010-01
- Publisher
- American Society for Biochemistry and Molecular Biology Inc.
- Citation
- Journal of Biological Chemistry, v. 285, NO. 4, Page. 2361-2367
- Abstract
- Transcription activation has been proposed to require both ubiquitylation and deubiquitylation of histone H2B. Here, we show that Lge1 (Large 1) is found in a complex containing Rad6.Bre1 and that it controls the recruitment of Bre1, a ubiquitin ligase, and Ubp8, a deubiquitylase, to promote ubiquitylation during the early steps in elongation. Chromatin immuno-precipitation experiments showed that Lge1 associates with promoter and coding regions of actively transcribed genes in a transcription-dependent manner. Disruption of Lge1 abolished ubiquitylation of histone H2B on lysine 123 and H3 methylation on lysines 4 and 79 and resulted in significant sensitivity to 6-azauracil and mycophenolic acid. In particular, in Lge1-deficient cells, Bre1 recruitment was attenuated, whereas recruitment of Ubp8 was facilitated. These alterations were coincident with changes in the interaction between Bre1.Ubp8 and RNA polymerase II phosphorylated at serine 5 of the C-terminal domain. We propose that Lge1 has a novel function in disrupting the balance between the recruitment of Bre1 and Ubp8, thus promoting transcription elongation.
- URI
- https://www.sciencedirect.com/science/article/pii/S0021925819637312?via%3Dihubhttps://repository.hanyang.ac.kr/handle/20.500.11754/185156
- ISSN
- 0021-9258;1083-351X
- DOI
- 10.1074/jbc.M109.039255
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
- Files in This Item:
- 1551_안성훈.pdfDownload
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