Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 안성훈 | - |
dc.date.accessioned | 2023-08-03T01:58:18Z | - |
dc.date.available | 2023-08-03T01:58:18Z | - |
dc.date.issued | 2010-01 | - |
dc.identifier.citation | Journal of Biological Chemistry, v. 285, NO. 4, Page. 2361-2367 | - |
dc.identifier.issn | 0021-9258;1083-351X | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0021925819637312?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/185156 | - |
dc.description.abstract | Transcription activation has been proposed to require both ubiquitylation and deubiquitylation of histone H2B. Here, we show that Lge1 (Large 1) is found in a complex containing Rad6.Bre1 and that it controls the recruitment of Bre1, a ubiquitin ligase, and Ubp8, a deubiquitylase, to promote ubiquitylation during the early steps in elongation. Chromatin immuno-precipitation experiments showed that Lge1 associates with promoter and coding regions of actively transcribed genes in a transcription-dependent manner. Disruption of Lge1 abolished ubiquitylation of histone H2B on lysine 123 and H3 methylation on lysines 4 and 79 and resulted in significant sensitivity to 6-azauracil and mycophenolic acid. In particular, in Lge1-deficient cells, Bre1 recruitment was attenuated, whereas recruitment of Ubp8 was facilitated. These alterations were coincident with changes in the interaction between Bre1.Ubp8 and RNA polymerase II phosphorylated at serine 5 of the C-terminal domain. We propose that Lge1 has a novel function in disrupting the balance between the recruitment of Bre1 and Ubp8, thus promoting transcription elongation. | - |
dc.description.sponsorship | This work was supported by Korea Research Foundation Grant KRF-2007521-C00200 and by Korea Science and Engineering Foundation Grant 2009-0079344 funded by the Korean government (Ministry of Education, Science, and Technology) (to S.H.A.). | - |
dc.language | en | - |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | - |
dc.subject | RNA-POLYMERASE-II | - |
dc.subject | UBIQUITINATION | - |
dc.subject | YEAST | - |
dc.subject | HISTONE H2B | - |
dc.subject | TERMINAL DOMAIN | - |
dc.subject | REGULATES H3 METHYLATION | - |
dc.subject | SAGA COMPLEX | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | SYSTEMATIC IDENTIFICATION | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.title | A Bre1-associated Protein, Large 1 (Lge1), Promotes H2B Ubiquitylation during the Early Stages of Transcription Elongation | - |
dc.type | Article | - |
dc.relation.no | 4 | - |
dc.relation.volume | 285 | - |
dc.identifier.doi | 10.1074/jbc.M109.039255 | - |
dc.relation.page | 2361-2367 | - |
dc.relation.journal | Journal of Biological Chemistry | - |
dc.contributor.googleauthor | Song, Young-Ha | - |
dc.contributor.googleauthor | Ahn, Seong Hoon | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 의약생명과학과 | - |
dc.identifier.pid | hoon320 | - |
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