PEGylated nanographene-mediated metallic nanoparticle clusters for surface enhanced Raman scattering-based biosensing
- Title
- PEGylated nanographene-mediated metallic nanoparticle clusters for surface enhanced Raman scattering-based biosensing
- Author
- 임동우
- Issue Date
- 2018-06
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- ANALYST, v. 143, NO. 11, Page. 2604-2615
- Abstract
- Surface-enhanced Raman scattering (SERS) is an optical spectroscopy technique that can detect a variety of analytes with high sensitivity and selectivity without any labels. Controlled clustering of metallic nanoparticles to prepare a new class of SERS nanotags is crucial for the ultra-sensitive detection of specific biological and chemical moieties because increased plasmonic hotspot junctions produce a greatly enhanced SERS signal. We report herein that controlled clustering of Au nanoparticles (AuNPs) was mediated by PEGylated nano-sized graphene (PNG) and that the PNG-induced AuNP clusters (PNG-AuNPCs) were highly sensitive SERS nanotags with colloidal stability for SERS-based biosensing. The AuNPs labeled with 4-mercaptopyridine as a Raman reporter were surface-modified with 1-aminomethylpyrene for the introduction of hydrophobic moieties, and were non-covalently complexed with PNG via p-p stacking and van der Waals forces. It resulted in the formation of PNG-AuNPCs that increased SERS intensity with an enhancement factor of 1.34 x 1011. The PNG induced a high degree of AuNP clustering by enhancing the non-covalent interactions between them, resulting in increased hotspot junctions at highly localized plasmonic centers. Furthermore, to show that the PNG-AuNPCs would serve as stable, reproducible, and highly sensitive SERS nanotags for biosensing, we formed sandwich-type immunocomplexes composed of the PNG-AuNPCs, immunoglobulin G (IgG) as the antigen, and magnetic beads. We found a linear relationship between SERS intensity and IgG concentration, with a limit of detection lower than 31.0 fM for IgG detection. Thus, the PNG-AuNPCs could be useful as SERS nanotags for highly sensitive SERS-based biosensing applications.
- URI
- https://pubs.rsc.org/en/content/articlelanding/2018/AN/C8AN00329Ghttps://repository.hanyang.ac.kr/handle/20.500.11754/183854
- ISSN
- 0003-2654;1364-5528
- DOI
- 10.1039/c8an00329g
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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