Determination of glycated albumin using boronic acid-derived agarose beads on paper-based devices
- Title
- Determination of glycated albumin using boronic acid-derived agarose beads on paper-based devices
- Author
- 성기훈
- Issue Date
- 2018-01
- Publisher
- AMER INST PHYSICS
- Citation
- BIOMICROFLUIDICS, v. 12, NO. 1, article no. 014111, Page. 1-8
- Abstract
- Self-monitoring of glycated albumin (GA), a useful glycemic marker, is an established method for preventing diabetes complications. Here, the paper-based lateral flow assay devices were developed for the sensitive detection of GA and the total human serum albumin (tHSA) in self-monitoring diabetes patients. Boronic acid-derived agarose beads were packed into a hole on a lateral flow channel. These well-coordinated agarose beads were used to capture GA through specific cis-diol interactions and to enhance the colorimetric signals by concentrating the target molecules. The devices exhibited large dynamic ranges (from 10 mu g/ml to 10 mg/ml for GA and from 10 mg/ml to 50 mg/ml for tHSA) and low detection limits (7.1 mu g/ml for GA and 4.7 mg/ml for tHSA), which cover the range of GA concentration in healthy plasma, which is 0.21-1.65 mg/ml (0.6%-3%). In determining the unknown GA concentrations in two commercial human plasma samples, the relative percentage difference between the values found by a standard ELISA kit and those found by our developed devices was 2.62% and 8.80%, which are within an acceptable range. The measurements of GA and tHSA were completed within 20 min for the total sample-to-answer diagnosis, fulfilling the demand for rapid analysis. Furthermore, the recovery values ranged from 99.4% to 110% in device accuracy tests. These results indicate that the developed paper-based device with boronic acid-derived agarose beads is a promising platform for GA and tHSA detection as applied to self-monitoring systems. Published by AIP Publishing.
- URI
- https://aip.scitation.org/doi/10.1063/1.5021395https://repository.hanyang.ac.kr/handle/20.500.11754/183533
- ISSN
- 1932-1058
- DOI
- 10.1063/1.5021395
- Appears in Collections:
- COLLEGE OF ENGINEERING SCIENCES[E](공학대학) > BIONANO ENGINEERING(생명나노공학과) > Articles
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