Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이성환 | - |
dc.date.accessioned | 2023-07-12T02:03:27Z | - |
dc.date.available | 2023-07-12T02:03:27Z | - |
dc.date.issued | 2008-05 | - |
dc.identifier.citation | Sensors and Actuators, B: Chemical, v. 132, NO. 1, Page. 327-333 | - |
dc.identifier.issn | 0925-4005 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0925400508000828?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/183130 | - |
dc.description.abstract | This paper presents the development of a PDMS-glass hybrid electro-immunosensing chip for real-time measurement of an antigen-antibody reaction through an electrical signal. Using a microfilter and microbeads, the antigen was easily immobilized in the detection zone where the microelectrodes were located. The immuno-reaction was detected by measuring the electrical resistance between microelectrodes using gold nanoparticles with silver enhancement. An immunoassay test with the developed chip was performed for the antigen of protein A, the specific first antibody of anti-protein A, the nonspecific first antibody of HBsAg monoclonal IgG, and the second antibody anti-rabbit IgG. The electro-immunosensing chip reduced the antigen-antibody reaction time to 10 min, thus reducing the overall assay time to about I h. The electrical resistance varied according to the concentration of the specific first antibody, the detection limit of which was 10 ng/ml. Compared to conventional enzyme-linked immunosorbent assays (ELISAs), the process of performing an immunoassay using the electro-immunosensing chip was relatively simple and required less time to complete. In addition, the electro-immunosensing chip required less sample volume. (C) 2008 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | No. R01-2003-000-10614-0 from the Basic Research Program of the Korea Science &Engineering Foundation. | - |
dc.language | en | - |
dc.publisher | Elsevier BV | - |
dc.subject | immunoassay | - |
dc.subject | electro-immunosensing | - |
dc.subject | microfilter | - |
dc.subject | microbead | - |
dc.subject | nanogold particle | - |
dc.subject | silver enhancement | - |
dc.subject | PDMS-glass microbiochip | - |
dc.title | Real-time immunoassay with a PDMS-glass hybrid microfilter electro-immuno sensing chip using nanogold particles and silver enhancement | - |
dc.type | Article | - |
dc.relation.no | 1 | - |
dc.relation.volume | 132 | - |
dc.identifier.doi | 10.1016/j.snb.2008.01.061 | - |
dc.relation.page | 327-333 | - |
dc.relation.journal | Sensors and Actuators, B: Chemical | - |
dc.contributor.googleauthor | Ko, Yong-Jun | - |
dc.contributor.googleauthor | Maeng, Joon-Ho | - |
dc.contributor.googleauthor | Ahn, Yoomin | - |
dc.contributor.googleauthor | Hwang, Seung-Yong | - |
dc.contributor.googleauthor | Cho, Nahm Gyoo | - |
dc.contributor.googleauthor | Lee, Seoung-Hwan | - |
dc.sector.campus | E | - |
dc.sector.daehak | 공학대학 | - |
dc.sector.department | 기계공학과 | - |
dc.identifier.pid | sunglee | - |
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