Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 최한곤 | - |
dc.date.accessioned | 2023-06-23T02:07:09Z | - |
dc.date.available | 2023-06-23T02:07:09Z | - |
dc.date.issued | 2022-12 | - |
dc.identifier.citation | LIFE SCIENCES, v. 311, article no. 121198, | - |
dc.identifier.issn | 0024-3205;1879-0631 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0024320522008980?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/182300 | - |
dc.description.abstract | Aims: Herein, we investigate the potential of levosulpiride-loaded nanostructured lipid carriers (LEVO-NLCs) for effective brain delivery with anti-psychotic and antidepressant effects. Main methods: Micro-emulsion method was used to prepare LEVO-NLCs, followed by its optimization using Design Expert (R), investigation of the particles properties and entrapment efficiency (%EE). Moreover, in-vitro release, in-vivo plasma and brain kinetic studies of LEVO-NLCs were executed. Anti-psychotic activity of LEVO-NLCs was accomplished in LPS-induced psychosis mice model. Additionally, expressions of neuro inflammatory mediators, neurodegeneration and neuro-inflammation in brain tissues was investigated. Key findings: The optimized LEVO-NLCs were rounded shaped nanoparticles (157.2 nm) with suitable zeta potential (-29.6 mV), low PDI (0.395) and high EE (83.67 %). No chemical interactions were found, however, the crystalline drug was changed to amorphous. LEVO-NLCs displayed sustained drug release behavior when compared with drug suspension. Moreover, a meaningfully higher AUC (106,642.27 +/- 876.44 ng.h/mL) and Cmax (38,534.72 +/- 2344.10 ng/mL) of the LEVO-NLCs in brain was observed as compared to the AUC (15,684.33 +/- 1005.49 ng.h/mL) and Cmax (7717.56 +/- 871.23 ng/mL) of LEVO-Suspension. Similar profiles of both the formulations were perceived in plasma pharmacokinetic studies. Furthermore, LEVO-NLCs exhibited a meaningfully improved anti-psychotic activity in LPS-induced psychosis mice model with reduced immobility time and enhanced struggling time. Likewise, treatment with LEVO-NLCs showed reduced levels of neuro inflammatory markers (p-NF-kappa B and COX-2) in LPS-induced mice. Additionally, no neuro-degeneration and neuroinflammation in brain tissues treated with LEVO-NLCs mice group was detected. Significance: These results concluded that NLCs may effectively be used for the brain delivery of various active pharmaceutical agents with enhanced biopharmaceutical performance. | - |
dc.description.sponsorship | This study was supported by the Higher Education Commission (HEC) Islamabad, Pakistan through its project Nos: HEC/SRGP/836/2016 and HEC/NRPU/R & D/No: 14604/2021. | - |
dc.language | en | - |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | - |
dc.subject | Levosulpiride | - |
dc.subject | Nanostructured lipid carriers | - |
dc.subject | Brain targeting | - |
dc.subject | LPS-induced psychosis and depression mice | - |
dc.subject | model | - |
dc.subject | Pharmacokinetics | - |
dc.title | Levosulpiride-loaded nanostructured lipid carriers for brain delivery with antipsychotic and antidepressant effects | - |
dc.type | Article | - |
dc.relation.volume | 311 | - |
dc.identifier.doi | 10.1016/j.lfs.2022.121198 | - |
dc.relation.journal | LIFE SCIENCES | - |
dc.contributor.googleauthor | Maqsood, Summaira | - |
dc.contributor.googleauthor | Din, Fakhar Ud | - |
dc.contributor.googleauthor | Khan, Saif Ullah | - |
dc.contributor.googleauthor | Elahi, Ehsan | - |
dc.contributor.googleauthor | Ali, Zakir | - |
dc.contributor.googleauthor | Jamshaid, Humzah | - |
dc.contributor.googleauthor | Zeb, Alam | - |
dc.contributor.googleauthor | Nadeem, Tunazza | - |
dc.contributor.googleauthor | Ahmed, Wajed | - |
dc.contributor.googleauthor | Khan, Salman | - |
dc.contributor.googleauthor | Choi, Han Gon | - |
dc.sector.campus | E | - |
dc.sector.daehak | 약학대학 | - |
dc.sector.department | 약학과 | - |
dc.identifier.pid | hangon | - |
dc.identifier.article | 121198 | - |
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