Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 신경훈 | - |
dc.date.accessioned | 2023-06-01T01:12:39Z | - |
dc.date.available | 2023-06-01T01:12:39Z | - |
dc.date.issued | 2007-06 | - |
dc.identifier.citation | Aquatic Toxicology, v. 83, NO. 2, Page. 104-115 | - |
dc.identifier.issn | 0166-445X;1879-1514 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0166445X07001154?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/181840 | - |
dc.description.abstract | We cloned and sequenced the full-length cDNA of a theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea. The open reading frame of N. succinea GST-T cDNA was 678 bp and encoded 226 amino acid residues. We generated recombinant N. succinea GST-T by expression in transformed Escherichia coli and studied the kinetic properties as well as the effects of inhibitors, pH, and temperature on N. succinea GST-T. GST-T expression was studied using real-time RT-PCR in response to exposure to the model oxidative stress-inducing agent, CuCl2. Copper induced a concentration-dependant increase in the expression of GST-T. Moreover, polychaetes collected from a heavily contaminated lake near an industrial complex showed significantly higher levels of GST-T expression. Interestingly, the site-collected polychaetes with the highest GST-T mRNA expression levels also showed the highest metallothioneins levels. These results suggest that GST-T in polychaetes may have an antioxidant role and that N. succinea GST-T expression may be a useful biomarker for exposure to environmental contaminants such as copper. Our findings provide a better understanding of the biochemical characteristics of N. succinea GST-T, and elucidate the potential role of GST-T in heavy metal-induced oxidative stress and as a biomarker for environmental contamination. (c) 2007 Elsevier B.V. All rights reserved. | - |
dc.description.sponsorship | Ansan Envi-ronmental Technology Development Center (2005) funded to Kyung-Hoon Shin. | - |
dc.language | en | - |
dc.publisher | Elsevier BV | - |
dc.subject | neanthes succinea | - |
dc.subject | glutathione S-transferases | - |
dc.subject | heavy metals | - |
dc.subject | metallothioneins | - |
dc.subject | oxidative stress | - |
dc.subject | biomarker | - |
dc.subject | polychaete | - |
dc.title | Molecular cloning, expression, biochemical characteristics, and biomarker potential of theta class glutathione S-transferase (GST-T) from the polychaete Neanthes succinea | - |
dc.type | Article | - |
dc.relation.no | 2 | - |
dc.relation.volume | 83 | - |
dc.identifier.doi | 10.1016/j.aquatox.2007.03.015 | - |
dc.relation.page | 104-115 | - |
dc.relation.journal | Aquatic Toxicology | - |
dc.contributor.googleauthor | Rhee, Jae-Sung | - |
dc.contributor.googleauthor | Lee, Young-Mi | - |
dc.contributor.googleauthor | Hwang, Dae-Sik | - |
dc.contributor.googleauthor | Won, Eun-Ji | - |
dc.contributor.googleauthor | Raisuddin, Sheikh | - |
dc.contributor.googleauthor | Shin, Kyung-Hoon | - |
dc.contributor.googleauthor | Lee, Jae-Seong | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 해양융합공학과 | - |
dc.identifier.pid | shinkh | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.