Whole genomic expression analysis of rat liver epithelial cells in response to phenytoin
- Title
- Whole genomic expression analysis of rat liver epithelial cells in response to phenytoin
- Author
- 황승용
- Keywords
- phenytoin; toxicogenomics; DNA chip; gene expression; rat liver epithelial cell
- Issue Date
- 2006-06
- Publisher
- 대한독성 유전단백체 학회
- Citation
- Molecular & Cellular Toxicology, v. 2, NO. 2, Page. 120-125
- Abstract
- Phenytoin is an anti-epileptic. It works by slowing down impulses in the brain that cause seizures. The recent microarray technology enables us to understand possible mechanisms of genes related to compounds which have toxicity in biological system. We have studied that the effect of a compound related to hepatotoxin in vitro system using a rat whole genome microarray. In this study, we have used a rat liver epithelial cell line WB-F344 and phenytoin as a hepatotoxin. WB-F344 was treated with phenytoin for 1 to 24 hours. Total RNA was isolated at times 1, 6 and 24h following treatment of phenytoin, and hybridized to the microarray containing about 22,000 rat genes. After analysis with clustering methods, we have identified a total of 1,455 differentially expressed genes during the time course. Interestingly, about 1,049 genes exhibited differential expression pattern in response to phenytoin in early time. Therefore, the identification of genes associated with phenytoin in early response may give important insights into various toxicogenomic studies in vitro system.
- URI
- http://koreascience.or.kr/article/JAKO200617317912019.jhttps://repository.hanyang.ac.kr/handle/20.500.11754/181696
- ISSN
- 1738-642X;2092-8467
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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