Separation-type multiplex polymerase chain reaction chip for detecting male infertility
- Title
- Separation-type multiplex polymerase chain reaction chip for detecting male infertility
- Author
- 황승용
- Keywords
- polymerase chain reaction; separable biochip; male infertility; multiple PCR chip
- Issue Date
- 2008-06
- Publisher
- IOP Publishing Ltd
- Citation
- Japanese Journal of Applied Physics, v. 47, NO. 6, Page. 5231-5235
- Abstract
- A novel polymerase chain reaction (PCR) biochip is presented in this paper. In this PCR chip, the glass substrate integrated with the microheater and microsensor is separable from the reaction chamber where the sample is injected, which now makes repeated reuse of the glass substrate possible. The heat transfer efficiency and target gene amplification of the proposed separable PCR chip was compared with that of the conventional united PCR chip. The results showed that the sex-determining Y chromosome (SRY) gene PCR for detecting male infertility was successfully performed in the separable chip. However, repeated multiplex PCR was successful for only two genes, SPGY I and SRY, but not for gene SY586. Future work is needed for a multiplex PCR with more than three genes.
- URI
- https://iopscience.iop.org/article/10.1143/JJAP.47.5231https://repository.hanyang.ac.kr/handle/20.500.11754/181680
- ISSN
- 0021-4922;1347-4065
- DOI
- 10.1143/JJAP.47.5231
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > ETC
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