Hyperpolarized NMR Analysis of Enzymatic Reaction: Extension of Observable Reaction Time by Deuterium Isotope Labeling
- Title
- Hyperpolarized NMR Analysis of Enzymatic Reaction: Extension of Observable Reaction Time by Deuterium Isotope Labeling
- Author
- 이영복
- Keywords
- Hyperpolarization; Nuclear magnetic resonance spectroscopy; Enzyme kinetics; Deuteration; Reaction intermediates
- Issue Date
- 2016-06
- Publisher
- WILEY-V C H VERLAG GMBH
- Citation
- BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v. 37, NO. 6, Page. 810.0-814.0
- Abstract
- Affording several orders of signal enhancement compared to conventional nuclear magnetic resonance (NMR), dynamic nuclear polarization (DNP) enables direct observation of the most common C-13 nuclei in organic chemistry and biochemistry at low concentrations. These nuclei have poor sensitivity due to low natural abundance and a low gyromagnetic ratio. Herein, we report an application of this technique to a multi-step enzymatic reaction. The main aim of this study was to demontrate the effect of deuteration on specific spins of interest for the real-time trypsin hydrolysis reaction of synthesized N-alpha-benzoyl-L-arginine methyl-d(3) ester (BAME-d(3)). Kinetic parameter and fit accuracy were analyzed in order to determine the influence of deuteration.
- URI
- https://onlinelibrary.wiley.com/doi/10.1002/bkcs.10773https://repository.hanyang.ac.kr/handle/20.500.11754/180707
- ISSN
- 0253-2964;1229-5949
- DOI
- 10.1002/bkcs.10773
- Appears in Collections:
- COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E](과학기술융합대학) > CHEMICAL AND MOLECULAR ENGINEERING(화학분자공학과) > Articles
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