Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 양철수 | - |
dc.date.accessioned | 2022-11-28T01:03:12Z | - |
dc.date.available | 2022-11-28T01:03:12Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.citation | Frontiers in Immunology, v. 13.0, article no. 862628, | - |
dc.identifier.issn | 1664-3224;1664-3224 | - |
dc.identifier.uri | https://www.frontiersin.org/articles/10.3389/fimmu.2022.862628/full | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/177540 | - |
dc.description.abstract | Mycobacterium tuberculosis (Mtb) is the causative pathogen of tuberculosis (TB), which manipulates the host immunity to ensure survival and colonization in the host. Mtb possess a unique family of proteins, named PE_PGRS, associated with Mtb pathogenesis. Thus, elucidation of the functions of PE_PGRS proteins is necessary to understand TB pathogenesis. Here, we investigated the role of PE_PGRS38 binding to herpesvirus-associated ubiquitin-specific protease (HAUSP, USP7) in regulating the activity of various substrate proteins by modulating their state of ubiquitination. We constructed the recombinant PE_PGRS38 expressed in M. smegmatis (Ms_PE_PGRS38) to investigate the role of PE_PGRS38. We found that Ms_PE_PGRS38 regulated the cytokine levels in murine bone marrow-derived macrophages by inhibiting the deubiquitination of tumor necrosis factor receptor-associated factor (TRAF) 6 by HAUSP. Furthermore, the PE domain in PE_PGRS38 was identified as essential for mediating TRAF6 deubiquitination. Ms_PE_PGRS38 increased the intracellular burden of bacteria by manipulating cytokine levels in vitro and in vivo. Overall, we revealed that the interplay between HAUSP and PE_PGRS38 regulated the inflammatory response to increase the survival of mycobacteria. | - |
dc.description.sponsorship | This work was supported by a National Research Foundation of Korea grant funded by the Korea government (MSIP) (grant no. 2019R1I1A2A01064237 and 2021R1A4A5032463) and the research fund of Hanyang University (HY-2021). | - |
dc.language | en | - |
dc.publisher | Frontiers Media S.A. | - |
dc.subject | Mycobacterium tuberculosis PE_PGRS38 | - |
dc.subject | Mycobacterium smegmatis | - |
dc.subject | HAUSP | - |
dc.subject | TRAF6 | - |
dc.subject | Macrophages | - |
dc.subject | Ubiquitination | - |
dc.title | PE_PGRS38 Interaction With HAUSP Downregulates Antimycobacterial Host Defense via TRAF6 | - |
dc.type | Article | - |
dc.relation.volume | 13.0 | - |
dc.identifier.doi | 10.3389/fimmu.2022.862628 | - |
dc.relation.journal | Frontiers in Immunology | - |
dc.contributor.googleauthor | Kim, Jae-Sung | - |
dc.contributor.googleauthor | Kim, Hyo Keun | - |
dc.contributor.googleauthor | Cho, Euni | - |
dc.contributor.googleauthor | Mun, Seok-Jun | - |
dc.contributor.googleauthor | Jang, Sein | - |
dc.contributor.googleauthor | Jang, Jichan | - |
dc.contributor.googleauthor | Yang, Chul-Su | - |
dc.sector.campus | E | - |
dc.sector.daehak | 과학기술융합대학 | - |
dc.sector.department | 분자생명과학과 | - |
dc.identifier.pid | chulsuyang | - |
dc.identifier.article | 862628 | - |
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