Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김기현 | - |
dc.date.accessioned | 2022-11-14T05:39:30Z | - |
dc.date.available | 2022-11-14T05:39:30Z | - |
dc.date.issued | 2021-08 | - |
dc.identifier.citation | Inorganic Chemistry Communications, v. 130, article no. 108745, Page. 1-7 | en_US |
dc.identifier.issn | 1387-7003;1879-0259 | en_US |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1387700321003002?via%3Dihub | en_US |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/176726 | - |
dc.description.abstract | This research describes the synthesis of a covalent bioconjugate of graphene quantum dots (GQDs) and glutaminase (10 units·mg−1) for electrochemical and photoluminescent (PL) detection of L-glutamine (Gln). As synthesized GQDs exhibited uniform size distribution, (average diameter ~5–10 nm, and discrete blue emission characteristics (λex = 391 ± 2 nm and λem = 480 ± 2 nm)). Accordingly, the immobilization of glutaminase over the surface of the GQDs was confirmed by the characteristic Fourier-transform infrared (FTIR) spectroscopy peaks of the amide bond at 1,703 and 1,390 cm−1. The conjugate was used to detect Gln using electrochemical and PL approaches on a parallel basis over a concentration range of 100–1,000 µM. Electrochemical analysis of Gln using cyclic voltammetry revealed a limit of detection (LOD) and limit of quantification (LOQ) values of 360.4 µM and 1,100 µM, respectively. In comparison, quantification of Gln based on the PL approach yielded a Stern-Volmer quenching constant, LOD, and LOQ values of 2.2 × 103 M−1, 9.42 µM, and 28.57 µM, respectively. As such, the PL spectroscopy-based analysis was seen to have better sensitivity for Gln quantification than the electrochemical analysis. The PL-based approach is a more recommendable option for Gln quantification with high sensitivity and rapidity. | en_US |
dc.description.sponsorship | This research was supported by SERB, Government of India, under the early career research program (No. YSS/2015/000212). KHK acknowledges support from the R&D Center for Green Patrol Technologies through the R&D for Global Top Environmental Technologies funded by the Ministry of the Environment (MOE Grant No: 2018001850001) and by a grant from the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, & Future Planning (Grant No: 2016R1E1A1A01940995). | en_US |
dc.language | en | en_US |
dc.publisher | Elsevier B.V. | en_US |
dc.subject | Cyclic voltammetry | en_US |
dc.subject | Glutaminase | en_US |
dc.subject | Glutamine | en_US |
dc.subject | Graphene quantum dots | en_US |
dc.subject | Sensing | en_US |
dc.title | Conjugate of graphene quantum dots and glutaminase for the sensing of L-glutamine: Electrochemical vs. fluorescent sensing approaches | en_US |
dc.type | Article | en_US |
dc.relation.volume | 130 | - |
dc.identifier.doi | 10.1016/j.inoche.2021.108745 | en_US |
dc.relation.page | 1-7 | - |
dc.relation.journal | Inorganic Chemistry Communications | - |
dc.contributor.googleauthor | Devi, Pooja | - |
dc.contributor.googleauthor | Kukkar, Deepak | - |
dc.contributor.googleauthor | Kaur, Manpreet | - |
dc.contributor.googleauthor | Thakur, Anupma | - |
dc.contributor.googleauthor | Kim, Ki-Hyun | - |
dc.contributor.googleauthor | Kukkar, Preeti | - |
dc.contributor.googleauthor | Kaur, Kamalpreet | - |
dc.contributor.googleauthor | Kaur, Harsimran | - |
dc.sector.campus | S | - |
dc.sector.daehak | 공과대학 | - |
dc.sector.department | 건설환경공학과 | - |
dc.identifier.pid | kkim61 | - |
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