Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 문효방 | - |
dc.date.accessioned | 2022-07-27T00:57:20Z | - |
dc.date.available | 2022-07-27T00:57:20Z | - |
dc.date.issued | 2021-04 | - |
dc.identifier.citation | ENVIRONMENTAL POLLUTION, v. 274, Page. 116586-116586 | en_US |
dc.identifier.issn | 0269-7491 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0269749121001640 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/171766 | - |
dc.description.abstract | Cresyl diphenyl phosphate (CDP), as a kind of aryl substituted organophosphate esters (OPEs), is commonly used as emerging flame retardants and plasticizers detected in environmental media. Due to the accumulation of CDP in organisms, it is very important to discover the toxicological mechanism and metabolic process of CDP. Hence, liver microsomes of crucian carps (Carassius carassius) were prepared for in vitro metabolism kinetics assay to estimate metabolism rates of CDP. After 140 min incubation, the depletion of CDP accounted for 58.1%-77.1% (expect 0.5 and 2 mu M) of the administrated concentrations. The depletion rates were best fitted to the Michaelis-Menten model (R-2 = 0.995), where maximum velocity (V-max) and Michaelis-Menten constant (K-m) were 12,700 +/- 2120 pmol min(-1).mg(-1) protein and 1030 +/- 212 mM, respectively. Moreover, the in vitro hepatic clearance (C-Lint) of CDP was 12.3 mu L min(-1).mg(-1) protein. Log K-ow and bioconcentration factor (BCF) of aryl-OPEs were both higher than those of alkyl- and chlorinated-OPEs, indicating that CDP may easily accumulate in aquatic organisms. The results made clear that the metabolism rate of CDP was greater than those of other OPEs detected in liver microsomes in previous research. This paper was first of its kind to comprehensively investigate the in vitro metabolic kinetics of CDP in fish liver microsomes. The present study might provide useful information to understand the environmental fate and metabolic processes of these kinds of substances, and also provide a theoretical basis for the ecological risk assessment of emerging contaminants. | en_US |
dc.description.sponsorship | This study was supported by National Natural Science Founda- tion of China (41773085, 41521003) and Major Science and Tech- nology Program for Water Pollution Control and Treatment (2017ZX07301005-001). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.subject | Cresyl diphenyl phosphate (CDP) | en_US |
dc.subject | Liver microsomes | en_US |
dc.subject | In vitro metabolism | en_US |
dc.subject | Kinetic assay | en_US |
dc.title | In vitro metabolic kinetics of cresyl diphenyl phosphate (CDP) in liver microsomes of crucian carp (Carassius carassius) | en_US |
dc.type | Article | en_US |
dc.relation.volume | 274 | - |
dc.identifier.doi | 10.1016/j.envpol.2021.116586 | - |
dc.relation.page | 116586-116586 | - |
dc.relation.journal | ENVIRONMENTAL POLLUTION | - |
dc.contributor.googleauthor | Yan, Zhenfei | - |
dc.contributor.googleauthor | Feng, Chenglian | - |
dc.contributor.googleauthor | Jin, Xiaowei | - |
dc.contributor.googleauthor | Liu, Daqing | - |
dc.contributor.googleauthor | Hong, Yajun | - |
dc.contributor.googleauthor | Qiao, Yu | - |
dc.contributor.googleauthor | Bai, Yingchen | - |
dc.contributor.googleauthor | Moon, Hyo-Bang | - |
dc.contributor.googleauthor | Qadeer, Abdul | - |
dc.contributor.googleauthor | Wu, Fengchang | - |
dc.relation.code | 2021003387 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MARINE SCIENCE AND CONVERGENCE ENGINEERING | - |
dc.identifier.pid | hbmoon | - |
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