Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 전희재 | - |
dc.date.accessioned | 2022-03-23T01:45:58Z | - |
dc.date.available | 2022-03-23T01:45:58Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 201, article no. 111959 | en_US |
dc.identifier.issn | 1011-1344 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S1011134420304097?via%3Dihub | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/169337 | - |
dc.description.abstract | Trigeminal ganglion (TG) neurons play an essential role in the sensory nerves of the face. Damaged TG neurons resulting from the accidental and non-intentional nerve lesions, commonly identified as neuropathic pain, which is known to cause intense pain and sensory abnormalities. For the treatment, surgical methods are conducted when the pharmacological treatment fails to provide satisfactory recovery. However, the process of surgery or drug intake can burden the patient or cause side effects. One of the logical choices of study becomes photo-biomodulation (PBM) referred to as therapeutic approaches based on the interactions of visible or near-infrared (NIR) photons with biomolecules inside cells or tissues. In this study, we constructed a PBM illumination setup to stimulate the cultured primary TG neurons and compared the growth morphology between the non-irradiated control group and irradiation group with NIR laser of 808 nm wavelength. In addition, we applied various radiant exposures of 1, 2, and 10 J/cm(2) with different pulse frequencies of 1, 10, and 100 Hz. We found that PBM could promote neurite growth of TG neurons, and it works at relatively low energy densities at 1 and 2 J/cm(2). The irradiation group in the pulsed wave mode with the frequency of 10 Hz was found to be the most effective when compared to other frequencies. Thus, PBM on TG neurons facilitated neuronal growth in vitro in a dose and frequency-dependent fashion. PBM may provide a potential therapeutic approach to treat damaged peripheral nerves. | en_US |
dc.description.sponsorship | The authors appreciate professor Sang-Mok Lee from HanGil Eye Hospital, Catholic Kwandong University College of Medicine for teaching us the in vitro primary culture of trigeminal ganglion neurons and professor Hyuk-Sang Kwon from GIST for helpful discussions throughout the project. This work was supported by Gwangju Institute of Science and Technology (GIST) Research Institute (GRI) grant funded by the GIST in 2020 and grants from the National Research Foundation of Korea (NRF) funded by the Korean government (MEST) (NRF-2019M3C7A1044964), the Brain Research Program through the NRF funded by the Ministry of Science, ICT & Future Planning (NRF-2017M3C7A1044964), and by the Bio & Medical Technology Development Program of NRF funded by the Korean government (MSIP & MOHW) (NRF-2015M3A9E2030125). | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER SCIENCE SA | en_US |
dc.subject | Primary cell culture | en_US |
dc.subject | Photobiomodulation | en_US |
dc.subject | Trigeminal ganglion neuron | en_US |
dc.subject | Near-infrared light | en_US |
dc.subject | Laser modulation mode (pulsed wave, continuous wave) | en_US |
dc.title | Neurite growth of trigeminal ganglion neurons in vitro with near-infrared light irradiation. | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1016/j.jphotobiol.2020.111959 | - |
dc.relation.journal | JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | - |
dc.contributor.googleauthor | Cho, Heejoo | - |
dc.contributor.googleauthor | Jeon, Hee-Jae | - |
dc.contributor.googleauthor | Park, Seonho | - |
dc.contributor.googleauthor | Park, Chul-Seung | - |
dc.contributor.googleauthor | Chung, Euiheon | - |
dc.relation.code | 2020052865 | - |
dc.sector.campus | S | - |
dc.sector.daehak | COLLEGE OF ENGINEERING[S] | - |
dc.sector.department | SCHOOL OF MECHANICAL ENGINEERING | - |
dc.identifier.pid | heejaic | - |
dc.identifier.orcid | https://orcid.org/0000-0003-1244-7793 | - |
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