Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 조성신 | - |
dc.date.accessioned | 2021-11-16T02:34:12Z | - |
dc.date.available | 2021-11-16T02:34:12Z | - |
dc.date.issued | 2020-05 | - |
dc.identifier.citation | ARTHRITIS RESEARCH & THERAPY, v. 22, no. 1, article no. 121 | en_US |
dc.identifier.issn | 1478-6362 | - |
dc.identifier.issn | 1478-6354 | - |
dc.identifier.uri | https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-020-02217-9 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/166264 | - |
dc.description.abstract | Background Ankylosing spondylitis (AS) is characteristically male-predominant, and progressive spinal ankylosis affects male patients more severely; however, the hormonal effects in males with AS are poorly understood. Methods In the present study, the regulatory effects of dutasteride, a 5-α reductase inhibitor that blocks the conversion of testosterone to dihydrotestosterone (DHT), were examined in curdlan-administered male SKG mice to determine spinal bone formation, bone metabolism-related markers, and interleukin (IL)-17A cytokine and T cell populations. In addition, the effects of DHT on primary osteoprogenitors from the facet joints of AS patients were assessed based on osteoblast-related parameters. DHT level was measured, and the correlation with modified Stoke Ankylosing Spondylitis Spinal Score (mSASSS) was analyzed in AS patients. Results In curdlan-administered SKG mice, dutasteride treatment resulted in an increased accumulation of hydroxyapatite in the spine which was positively correlated with serum IL-17A levels. In the analysis of bone metabolism-related molecules, a decrease in sclerostin levels was observed in the sera in the dutasteride group. Continuous exposure to DHT resulted in fewer calcium deposits in AS osteoprogenitors during osteoblast differentiation. DHT-treated AS osteoprogenitors showed decreased osteocalcin and increased DKK1 and SOST1 mRNA expression, supporting the results of the in vivo experiments. Treatment with dutasteride upregulated bone formation in the spine of curdlan-administered SKG mice and DHT treatment downregulated osteoblast differentiation in vitro. Conclusions Treatment with dutasteride affected the bone formation in the spine of curdlan-treated SKG mice, and DHT treatment attenuated osteoblast differentiation in vitro. Therefore, contrary to what could be expected if osteoblasts contributed to spinal ankylosis, DHT inhibition might increase rather than decrease the progression of spinal ankylosis despite the higher levels of DHT observed in many AS patients. | en_US |
dc.description.sponsorship | This research was supported by grants from the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1A6A3A11034394 to SJ, 2019R1F1A1059736 to Y-GK, and 2016R1A2B4008606 and 2019R1A2C2004214 to T-HK). It was also supported by a Korea Health Technology R&D grant through the Korea Health Industry Development Institute (KHIDI), which is funded by the Ministry of Health & Welfare, Republic of Korea (HI17C0888 to T-HK). | en_US |
dc.language.iso | en | en_US |
dc.publisher | BMC | en_US |
dc.subject | Ankylosing spondylitis | en_US |
dc.subject | Spinal ankylosis | en_US |
dc.subject | Dutasteride | en_US |
dc.subject | Curdlan-administered SKG mice | en_US |
dc.subject | Dihydrotestosterone | en_US |
dc.subject | Osteoprogenitors | en_US |
dc.subject | Osteoblastic activity | en_US |
dc.title | Effects of dihydrotestosterone on osteoblast activitiy in curdlan-administered SKG mice and osteoprogenitor cells in patients with ankylosing spondylitis | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1186/s13075-020-02217-9 | - |
dc.relation.page | 1-7 | - |
dc.relation.journal | ARTHRITIS RESEARCH & THERAPY | - |
dc.contributor.googleauthor | Jo, Sungsin | - |
dc.contributor.googleauthor | Lee, Eun-Ju | - |
dc.contributor.googleauthor | Nam, Bora | - |
dc.contributor.googleauthor | Kang, Juyeon | - |
dc.contributor.googleauthor | Lee, Seunghun | - |
dc.contributor.googleauthor | Youn, Jeehee | - |
dc.contributor.googleauthor | Park, Ye-Soo | - |
dc.contributor.googleauthor | Kim, Yong-Gil | - |
dc.contributor.googleauthor | Kim, Tae-Hwan | - |
dc.relation.code | 2020048263 | - |
dc.sector.campus | S | - |
dc.sector.daehak | RESEARCH INSTITUTE[S] | - |
dc.sector.department | RHEUMATISM CENTER | - |
dc.identifier.pid | joejo0517 | - |
dc.identifier.researcherID | AAL-3659-2021 | - |
dc.identifier.orcid | https://orcid.org/0000-0003-3034-5029 | - |
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