Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김종호 | - |
dc.date.accessioned | 2021-10-29T01:54:42Z | - |
dc.date.available | 2021-10-29T01:54:42Z | - |
dc.date.issued | 2009-08 | - |
dc.identifier.citation | ANALYTICAL BIOCHEMISTRY, v. 391, no. 1, page. 24-30 | en_US |
dc.identifier.issn | 0003-2697 | - |
dc.identifier.uri | https://www.sciencedirect.com/science/article/pii/S0003269709003236 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/166005 | - |
dc.description.abstract | This article presents a prototype of a surface-enhanced Raman spectroscopy (SERS)-encoded magnetic bead of 8 mu m diameter. The core part of the bead is composed of a magnetic nanoparticle (NP)-embedded sulfonated polystyrene bead. The outer part of the bead is embedded with Ag NPs on which labeling molecules generating specific SERS bands are adsorbed. A silica shell is fabricated for further bioconjugation and protection of SERS signaling. Benzenethiol, 4-mercaptotoluene, 2-naphthalenethiol, and 4-aminothiophenol are used as labeling molecules. The magnetic SERS beads are used as Substrates for protein sensing and screening with easy handling. As a model application, streptavidin-bound magnetic SERS beads are used to illustrate selective separation in a flow cytometry system, and the screened beads are spectrally recognized by Raman spectroscopy. The proposed magnetic SERS beads are likely to be used as a versatile solid support for protein sensing and screening in multiple assay technology. (C) 2009 Elsevier Inc. All rights reserved. | en_US |
dc.description.sponsorship | This work was supported by the Nano-Systems Institute–National Core Research Center (NSI–NCRC) program, the Nano-Bioelectronics and Systems Research Center of the Korea Science and Engineering Foundation (KOSEF), a grant of the Korea Health 21 R&D Project of the Ministry of Health and Welfare (A050432), and the Brain Korea 21 Program supported by the Ministry of Education and Human Resources Development. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | en_US |
dc.subject | Silver nanoparticles | en_US |
dc.subject | Optically active materials | en_US |
dc.subject | Magnetic materials | en_US |
dc.subject | Polymeric materials | en_US |
dc.subject | Surface-enhanced Raman scattering | en_US |
dc.title | Protein separation and identification using magnetic beads encoded with surface-enhanced Raman spectroscopy | en_US |
dc.type | Article | en_US |
dc.relation.no | 1 | - |
dc.relation.volume | 391 | - |
dc.identifier.doi | 10.1016/j.ab.2009.05.005 | - |
dc.relation.page | 24-30 | - |
dc.relation.journal | ANALYTICAL BIOCHEMISTRY | - |
dc.contributor.googleauthor | Jun, Bong-Hyun | - |
dc.contributor.googleauthor | Noh, Mi Suk | - |
dc.contributor.googleauthor | Kim, Gunsung | - |
dc.contributor.googleauthor | Kang, Homan | - |
dc.contributor.googleauthor | Kim, Jong-Ho | - |
dc.contributor.googleauthor | Chung, Woo-Jae | - |
dc.contributor.googleauthor | Kim, Min-Soo | - |
dc.contributor.googleauthor | Kim, Yong-Kweon | - |
dc.contributor.googleauthor | Cho, Myung-Haing | - |
dc.contributor.googleauthor | Jeong, Dae Hong | - |
dc.relation.code | 2009200597 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF ENGINEERING SCIENCES[E] | - |
dc.sector.department | DEPARTMENT OF MATERIALS SCIENCE AND CHEMICAL ENGINEERING | - |
dc.identifier.pid | kjh75 | - |
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