Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 김진기 | - |
dc.date.accessioned | 2021-09-07T06:58:50Z | - |
dc.date.available | 2021-09-07T06:58:50Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | Journal of Pharmaceutical Investigation, v. 50, Issue. 4, Page. 373-381 | en_US |
dc.identifier.issn | 2093-6214 | - |
dc.identifier.issn | 2093-5552 | - |
dc.identifier.uri | https://link.springer.com/article/10.1007%2Fs40005-019-00462-y | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/164881 | - |
dc.description.abstract | Purpose The present study aims to investigate the neuroprotective effects of carnosine-entrapped elastic liposomes (CAR-ELs) against cerebral ischemia. Methods CAR-ELs were prepared by extrusion method using egg phosphatidylcholine (eggPC) as a phospholipid and Tween 80 (TW80) as an edge activator (eggPC:TW80 = 8:2, w/w). The prepared CAR-ELs were purified by centrifugal ultrafiltration followed by characterization for particle size, polydispersity index, zeta potential and entrapment efficiency. The elasticity of CAR-ELs, the most distinct feature of elastic liposomes, was determined using a stainless steel pressure filter and compared with that of conventional liposomes. In vivo neuroprotective effects of CAR-ELs were evaluated in cerebral ischemia induced by permanent middle cerebral artery occlusion (pMCAO) in rats. CAR-ELs (250 mg/kg of CAR) were intravenously administered 20 min before pMCAO and 6 h after pMCAO, respectively. The infarct volume in brain was measured by staining with 2,3,5-triphenyltetrazolium chloride after 24 h of cerebral ischemia. Results CAR-ELs showed nanometric particle size near 100 nm and homogeneous distribution with polydispersity index below 0.1. The elasticity of CAR-ELs was 2-fold higher than that of conventional liposomes. The brain ischemia was successfully developed with pMCAO as indicated by highly infarcted hemisphere (~ 50%) in saline-treated rats. The pre-treatment with CAR-ELs significantly reduced infarct volume (7.9%) compared with CAR solution (19.1%)- and saline (50.8%)-pretreated rats. CAR solution, however, showed better neuroprotective effects than CAR-ELs when administered 6 h after ischemia induction. Conclusion The pre-treatment with CAR-ELs could be promising nanocarrier-based neuroprotective therapeutics against ischemic stroke. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Netherlands | en_US |
dc.title | Neuroprotective effects of carnosine-loaded elastic liposomes in cerebral ischemia rat model | en_US |
dc.type | Article | en_US |
dc.relation.no | 4 | - |
dc.relation.volume | 50 | - |
dc.identifier.doi | 10.1007/s40005-019-00462-y | - |
dc.relation.page | 373-381 | - |
dc.relation.journal | Journal of Pharmaceutical Investigation | - |
dc.contributor.googleauthor | Zeb, Alam | - |
dc.contributor.googleauthor | Cha, Ji-Hye | - |
dc.contributor.googleauthor | Noh, Ah Reum | - |
dc.contributor.googleauthor | Qureshi, Omer Salman | - |
dc.contributor.googleauthor | Kim, Kyoung-Won | - |
dc.contributor.googleauthor | Choe, Yeong-Hwan | - |
dc.contributor.googleauthor | Shin, Donggeun | - |
dc.contributor.googleauthor | Shah, Fawad Ali | - |
dc.contributor.googleauthor | Majid, Arshad | - |
dc.contributor.googleauthor | Bae, Ok-Nam | - |
dc.contributor.googleauthor | Kim, Jin-Ki | - |
dc.relation.code | 2020014809 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | jinkikim | - |
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