Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김철영 | - |
dc.date.accessioned | 2021-09-07T06:50:13Z | - |
dc.date.available | 2021-09-07T06:50:13Z | - |
dc.date.issued | 2020-07 | - |
dc.identifier.citation | MOLECULES, v. 25, Issue. 13, Article no. 3077, 10pp | en_US |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://www.mdpi.com/1420-3049/25/13/3077 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/164861 | - |
dc.description.abstract | A comprehensive linear gradient solvent system for centrifugal partition chromatography (CPC) was developed for the bioassay-guided isolation of natural compounds. The gradient solvent system consisted of three different ternary biphasic solvents types:n-hexane-acetonitrile-water (10:2:8,v/v), ethyl acetate-acetonitrile-water (10:2:8,v/v), and water-saturatedn-butanol-acetonitrile-water (10:2:8,v/v). The lower phase of then-hexane-acetonitrile-water (10:2:8,v/v) was used as the stationary phase, while its upper phase, as well as ethyl acetate-acetonitrile-water (10:2:8), and water-saturatedn-butanol-acetonitrile-water (10:2:8,v/v) were pumped to generate a linear gradient elution, increasing the mobile phase polarity. We used the gradient CPC to identify antioxidant response elements (AREs), inducing compounds fromCentipeda minima, using an ARE-luciferase assay in HepG2 cells, which led to the purification of the active molecules 3-methoxyquercetin and brevilin A. The developed CPC solvent systems allow the separation and isolation of compounds with a wide polarity range, allowing active molecule identification in the complex crude extract of natural products. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | MDPI | en_US |
dc.subject | centrifugal partition chromatography | en_US |
dc.subject | comprehensive linear gradient elution | en_US |
dc.subject | bioactivity-guided isolation | en_US |
dc.subject | 3-methoxyquercetin | en_US |
dc.subject | brevilin A | en_US |
dc.subject | Centipeda minima | en_US |
dc.title | Bioassay-Guided Separation ofCentipeda minimaUsing Comprehensive Linear Gradient Centrifugal Partition Chromatography | en_US |
dc.type | Article | en_US |
dc.relation.no | 13 | - |
dc.relation.volume | 25 | - |
dc.identifier.doi | 10.3390/molecules25133077 | - |
dc.relation.page | 1-10 | - |
dc.relation.journal | MOLECULES | - |
dc.contributor.googleauthor | Kim, Ji Hoon | - |
dc.contributor.googleauthor | Jung, Eun Ju | - |
dc.contributor.googleauthor | Lee, Yun Jung | - |
dc.contributor.googleauthor | Gao, En Mei | - |
dc.contributor.googleauthor | Syed, Ahmed Shah | - |
dc.contributor.googleauthor | Kim, Chul Young | - |
dc.relation.code | 2020053399 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF PHARMACY[E] | - |
dc.sector.department | DEPARTMENT OF PHARMACY | - |
dc.identifier.pid | chulykim | - |
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