Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최장식 | - |
dc.date.accessioned | 2021-07-07T07:02:34Z | - |
dc.date.available | 2021-07-07T07:02:34Z | - |
dc.date.issued | 2020-03 | - |
dc.identifier.citation | SMALL, v. 16, no. 21, article no. 1907674 | en_US |
dc.identifier.issn | 1613-6810 | - |
dc.identifier.issn | 1613-6829 | - |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/full/10.1002/smll.201907674 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/162711 | - |
dc.description.abstract | Understanding the interactions between nanoparticles (NPs) and human immune cells is necessary for justifying their utilization in consumer products and biomedical applications. However, conventional assays may be insufficient in describing the complexity and heterogeneity of cell-NP interactions. Herein, mass cytometry and single-cell RNA-sequencing (scRNA-seq) are complementarily used to investigate the heterogeneous interactions between silver nanoparticles (AgNPs) and primary immune cells. Mass cytometry reveals the heterogeneous biodistribution of the positively charged polyethylenimine-coated AgNPs in various cell types and finds that monocytes and B cells have higher association with the AgNPs than other populations. scRNA-seq data of these two cell types demonstrate that each type has distinct responses to AgNP treatment: NRF2-mediated oxidative stress is confined to B cells, whereas monocytes show Fc gamma-mediated phagocytosis. Besides the between-population heterogeneity, analysis of single-cell dose-response relationships further reveals within-population diversity for the B cells and naive CD4(+) T cells. Distinct subsets having different levels of cellular responses with respect to their cellular AgNP doses are found. This study demonstrates that the complementary use of mass cytometry and scRNA-seq is helpful for gaining in-depth knowledge on the heterogeneous interactions between immune cells and NPs and can be incorporated into future toxicity assessments of nanomaterials. | en_US |
dc.description.sponsorship | This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation of Korea funded by the Ministry of Science & ICT (Grant Agreement No. 2017M3A9G8084539) and by Korea Basic Science Institute (National Research Facilities and Equipment Center) funded by the Ministry of Education (Grant Agreement No. 2019R1A6C1030014). It was also supported by the International Cooperation Research Program of the National Research Foundation of Korea funded by the Ministry of Science & ICT (Grant Agreement No. 2019K1A3A1A78112959) as a part of the European Union’s Horizon 2020 NanoSolveIT Project (Grant Agreement No. 814572). Blood samples used in this study were obtained from Hanyang University Hospital (Seoul, Republic of Korea) with informed consent from the donors and approval from the Institutional Review Board (No. HYUH 2018-09-005-004). Mass cytometry experiments were performed on the Helios instrument at the Biotechnology Research Center in POSTECH, Republic of Korea. The authors would like to thank Prof. Young Tae Chang and Dr. Jong Jin Kim (POSTECH, Republic of Korea) for the use of Helios system for this research. | en_US |
dc.language.iso | en | en_US |
dc.publisher | WILEY-V C H VERLAG GMBH | en_US |
dc.subject | heterogeneity | en_US |
dc.subject | mass cytometry | en_US |
dc.subject | peripheral blood mononuclear cells | en_US |
dc.subject | RNA sequencing | en_US |
dc.subject | silver nanoparticles | en_US |
dc.subject | single cells | en_US |
dc.title | Mass Cytometry and Single‐Cell RNA‐seq Profiling of the Heterogeneity in Human Peripheral Blood Mononuclear Cells Interacting with Silver Nanoparticles | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1002/smll.201907674 | - |
dc.relation.page | 1-13 | - |
dc.relation.journal | SMALL | - |
dc.contributor.googleauthor | Ha, My Kieu | - |
dc.contributor.googleauthor | Kwon, Sook Jin | - |
dc.contributor.googleauthor | Choi, Jang-Sik | - |
dc.contributor.googleauthor | Nguyen, Nguyen Thanh | - |
dc.contributor.googleauthor | Song, Jaewoo | - |
dc.contributor.googleauthor | Lee, Yangsoon | - |
dc.contributor.googleauthor | Kim, Young-Eun | - |
dc.contributor.googleauthor | Shin, Incheol | - |
dc.contributor.googleauthor | Nam, Jin-Wu | - |
dc.contributor.googleauthor | Yoon, Tae Hyun | - |
dc.relation.code | 2020047591 | - |
dc.sector.campus | S | - |
dc.sector.daehak | RESEARCH INSTITUTE[S] | - |
dc.sector.department | INSTITUTE FOR MATERIALS DESIGN | - |
dc.identifier.pid | gksakdma0529 | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.