Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정일엽 | - |
dc.date.accessioned | 2021-04-26T04:50:12Z | - |
dc.date.available | 2021-04-26T04:50:12Z | - |
dc.date.issued | 2000-06 | - |
dc.identifier.citation | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, v. 31, no. 3, page. 245-248 | en_US |
dc.identifier.uri | https://iubmb.onlinelibrary.wiley.com/doi/full/10.1042/BA19990101 | - |
dc.identifier.uri | https://repository.hanyang.ac.kr/handle/20.500.11754/161677 | - |
dc.description.abstract | A cDNA encoding mature epidermal growth factor (EGF) was isolated and cloned into a pQE30 vector in which the His(6)-tagged EGF was expressed. pH-stat feeding of concentrated medium at the time of isopropyl beta-D-thiogalactoside induction and slug-feedings of the enriched medium during the induction resulted in a higher cell density and specific expression. Using a simple refolding protocol that consisted of 1 mM L-cysteine addition for a 1-h reduction followed by 5 mM L-cystine addition for oxidative refolding, we were able to convert nearly all EGF monomers into the oxidized form. Also, there folding aggregate was converted into the monomeric form. Approx. 50% overall yield was obtained from the dissolved inclusion bodies to a single peak under FPLC. We hope that the result of this study may provide information that is useful for the scale-up of the recombinant human EGF production process. | en_US |
dc.description.sponsorship | This study was supported financially by a Biochemical Engineering Research Grant from the Ministry of Education of Korea (96‐G‐7). J.Y.L. is grateful to the Graduate School of Advanced Materials and Chemical Engineering at Hanyang University for a research fellowship. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Portland Press Ltd | en_US |
dc.subject | L-cystine, | en_US |
dc.subject | fed-batch | en_US |
dc.subject | recombinant EGF | en_US |
dc.subject | refolding | en_US |
dc.title | Scale-up process for expression and renaturation of recombinant human epidermal growth factor from Escherichia coli inclusion bodies. | en_US |
dc.type | Article | en_US |
dc.identifier.doi | 10.1042/BA19990101 | - |
dc.relation.journal | BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | - |
dc.contributor.googleauthor | Lee, JY | - |
dc.contributor.googleauthor | Yoon, CS | - |
dc.contributor.googleauthor | Chung, IY | - |
dc.contributor.googleauthor | Lee, YS | - |
dc.contributor.googleauthor | Lee, EK | - |
dc.relation.code | 2009201354 | - |
dc.sector.campus | E | - |
dc.sector.daehak | COLLEGE OF SCIENCE AND CONVERGENCE TECHNOLOGY[E] | - |
dc.sector.department | DEPARTMENT OF MOLECULAR AND LIFE SCIENCE | - |
dc.identifier.pid | iychu | - |
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